FoxP2 brainstem neurons project to sodium appetite regulatory sites》.pdfVIP

Journal of Chemical Neuroanatomy 42 (2011) 1–23 Contents lists available at ScienceDirect Journal of Chemical Neuroanatomy jo ur n al ho mep ag e: www .elsevier .c om/lo cate/jc h emn eu Review FoxP2 brainstem neurons project to sodium appetite regulatory sites Jung-Won Shin 1, Joel C. Geerling, Matthew K. Stein, Rebecca L. Miller, Arthur D. Loewy * Department of Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, MO 63110, USA A R T I C L E I N F O A B S T R A C T Article history: The transcription factor Forkhead box protein 2 (FoxP2) is expressed in two cell groups of the brainstem Received 30 March 2011 that have been implicated in sodium appetite regulation: the pre-locus coeruleus (pre-LC) and Received in revised form 6 May 2011 parabrachial nucleus – external lateral-inner subdivision (PBel-inner). Because the connections of these Accepted 7 May 2011 two groups are unknown, neuroanatomical tracing methods were used to define their central projections. Available online 13 May 2011 The pre-LC outputs were first analyzed using an anterograde axonal tracer – Phaseolus vulgaris leucoagglutinin (PHAL) to construct a brain map. Next, we examined whether the FoxP2 immunoreactive Keywords: (FoxP2+) neurons of the pre-LC con