Exclusive Association of Paraoxonase 1 with High-Density Lipoprotein Particles in Apolipoprotein A-I Deficiency》.pdfVIP

Biochemical and Biophysical Research Communications 289, 395–401 (2001) doi:10.1006/bbrc.2001.5985, available online at on Exclusive Association of Paraoxonase 1 with High-Density Lipoprotein Particles in Apolipoprotein A-I Deficiency Hiroshi Noto,* Yoshiaki Hashimoto,† Hiroaki Satoh,* Masumi Hara,* Naoyuki Iso-o,* Masako Togo,* Satoshi Kimura,* and Kazuhisa Tsukamoto*,1 *Department of Metabolic Diseases and †Department of Clinical Laboratory Medicine, Graduate School of Medicine, University of Tokyo, Tokyo, Japan Received October 19, 2001 risk factors for CHD, several epidemiological studies Paraoxonase1 (PON1) is a high-density lipoprotein have identified a low plasma high-density lipoprotein (HDL)-associated protein which removes peroxidized (HDL) cholesterol level is the strongest one (1–4). lipids from lipoproteins. It has been proposed that The reverse cholesterol transport, the mechanism apolipoprotein A-I (apoA-I) is an important determi- that recruits excess cholesterol from the peripheral nant for its stabilization on HDL. However, little is tissues and transports it to the liver, is an established known about its existence and activity in an apoA-I- antiatherogenic property of HDL. Another potential deficient state in humans. To characterize the nature antiatherogenic property of HDL is its capacity to pro- of PON1 in apoA-I deficiency, we investigated PON1 in tect low-density lipoprotein (LDL) from lipid peroxida- an apoA-I-deficient patient. When serum was analyzed on fast protein liquid chromatography, PON1 protein tion, which is the crucial first step for atherogenesis in