肾炎滴丸的制备与药效学考察.pdfVIP

医药导报2015年5月第34卷第5期 ·617 · 肾炎滴丸的制备与药效学考察 于丽秀,杨玉,何秋月,陈东生 (华中科技大学同济医学院附属协和医院药学部,武汉　 430022) 摘　 要　 目的　 制备肾炎滴丸并考察其药效学。 方法　 正交实验优化其肾炎滴丸制备工艺;采用切除5/ 6 肾的方 法建立慢性肾衰竭模型,考察肾炎滴丸对慢性肾衰竭大鼠尿蛋白及肾脏组织结构的影响。 结果　 肾炎滴丸的最佳制备 工艺为PEG4000 ：PEG6000=2 ：1,药物 ：基质=1 ：3,滴制温度80 ℃,滴距7 cm;药效学结果显示:与模型对照组比较, 肾炎滴丸组与肾衰丸组大鼠24 h尿蛋白水平均明显降低(P0.01),肾炎滴丸组降低更为显著(P0.05);病理组织检查 结果显示治疗组大鼠肾小管基本恢复正常,间质有少量炎症细胞浸润,肾间质纤维组织增生得到较好抑制。 结论　 肾炎 滴丸制备工艺稳定,且对慢性肾衰竭有较好的作用,但最佳治疗剂量还有待进一步验证。 关键词　 肾炎滴丸;肾衰竭,慢性;尿蛋白 中图分类号　 R286;R283　 　 　 文献标识码　 A　 　 　 文章编号　 1004-0781(2015)05-0617-04 Preparation of Nephritis Dripping Pills and Its Pharmacodynamic Studies YU Lixiu, YANG Yu, HE Qiuyue, CHEN Dongsheng(Department of Pharmacy, Union Hospital, Tongji Medical College,Huazhong University of Science and Technology,Wuhan430022,China) ABSTRACT　 Objective　 To prepare nephritis dripping pills and study its pharmacodynamic effects.　 Methods　 The nephritis dripping pillswereself-made,andthepreparationwasoptimizedbyusingorthogonalmethod.Themodelofchronicrenal failure was established through resection of 5/ 6 kidney,the effect of nephritis dripping pills on rats was investigated by urinary protein and renal pathology analysis. 　 Results　 The optimal preparation for nephritis dripping pills was as follows:taking PEG4000 ：PEG6000 =2 ：1, drug ：matrix = 1 ：3,setting temperature at 80 ℃ and dropping distance as 7 cm. The pharmacodynamicresultsshowedthat:comparedwiththemodelcontrolgroups,thenephritisdroppingpillsandrenalfailurepills significantly reduced the24 h urine protein levels (P0.01),and the nephritis dripping pills were significant superior to renal failure pills (P0.05).The histopathological results showed that the renal tubular of treated groups remitted to normal,renal interstitial presen