增强CEBPε表达对人粒-单核性白血病细胞U-937分化的作用.pdf

!#$ 癌症 a a )Z Chinese JournaI of Cancer 2006 25 l1 1368-1373 基础研究 增强 表达对人粒 单核性白血病细胞 C/ EBP! - U-937 分化的作用 蔡 蓉 戴冰冰 李 珂 王红洁 许伟榕 王家敏 卢 健 , , , , , , Promotive Effect of C/ EBP! Overexpression on Differentiation of Human Myelomonocytic Leukemia Cell Line U-937 CA I Rong , DA I Bing-Bing , LI Ke , WANG Hong-Jie , XU Wei-Rong , WANG Jia-min , LU Jian !ABSTRACT BACKGROUND OBJECTIVE # CCAAT-enhancer binding protein ! C/ EBP! is a kind of nuclear transcriptional factor expressed predominantly in myeloid cells , and may be a critical regulator of myeloid differentiation , which can activate the transcription of a subset of myeloid- specific genes . This study was to detect the cell-specific expression of C/ EBP! , and to investigate the effect of C/ EBP! overexpression on c-myc expression , cell cycle distribution , and cell differentiation of human 上海交通大学医学院 , myelomonocytic leukemia cell line U-937. METHODS # The expression of C/ 生物化学与分子生物学教研室 , EBP! in 5 hematopoietic cell lines U-937 , NB4 , HL-60 , K-562 and Jurkat T 上海 , 200025 cell lines was tested by semi