Stathmin基因表达和肿瘤化疗药物敏感性的关系-副本.pdfVIP

784 ( J Fourth M ilM ed Un iv) 2005; 26( 9) htt : / /journal. fmm u. edu. cn : 1000-2790( 2005) 09-0784-05 Stathmin 井晓荣, 刘 丽, 赵 辉, 张惠中 ( , 710038) Relationship between expression of : 8 ( vincristine, VCR)( docetaxel, DOC) Stathmin gene in cancer cells and its Stathm in. : sensitivity to chemotherapeutic drugs MTT, J ING X iao-Rong, LIU L i, ZHA O H ui, ZHAN G H ui-Zhong , 8; De artm ent of C lin ical Diagnosis, Tangdu H os ita,l Fourth M ili- PCR Stathm in . tary M ed icalUn iversity, Xian 710038, Ch ina : 8 VCR ( ) : 9607, 58% , 9901, 56% ; H ela, AbstractAIM: To investgi ate the sensitivity ofeight kinds 37% ; HH CC, 34% ; A549, 31% ; of cancercells to two kinds ofchemotherapeutic drugs: Vin- H e -2, 17% ; MKN45, 16% ; 7721, 15% . cristine andDocetaxeland to study the real tionshpi between DOC : 9607, 48% ; H ela, 46% ; 9901, the sensitiv ity and the expressionofStathm ni gene in these 44% ; H e -2, 34% ; A549, 31% ; MKN45, 15% ; HH CC, 12% ; cells. METH DS: The chemosensitivity of the eight kinds 7721, 3% ; Stathm in of cancerce lls toV incristine (V R) and Docetaxel(DO ) , . was detected by MTT colormi etric assay and morphool gical : Statnm in observation. Real tmi e P R technologywas used to exam- , Statnm in ine the expressoi n of Stathmin gene in these ce lls. . RESULTS: The inhbi itoi n rates of the e gi ht kinds of cancer 肿瘤细胞;长春新碱; 泰索帝; MTT试验; 抑制率; 实 cells by V R and DO were respectively 58% and 48% for 时定量 PCR human osteosarcoma cells line Sosp-9607 ( 9607 ), 56% R73 A and 44% for human osteosarcoma cells line Sosp-9901 (9901), 37% and 46% for cervical cancer cells line Hela 0 ( Hela), 34% and 12% for human hepatocellular carcinoma