HPLC法测定烟草和烟草制品中马来酰肼残留.pdfVIP

鐑熻崏绉戞妧/TobaccoScienceTechnology 鐑熻崏鍖栧/Tobacco Chemistry 2011 骞寸 12 鏈燂紙鎬荤293 鏈燂級 HPLC娉曟祴瀹氱儫鑽夊強鐑熻崏鍒跺搧涓┈鏉ラ叞鑲兼畫鐣 寮犳椽闈烇紝杈圭収闃筹紝鍞愮翰宀紝鑳℃竻婧 涓浗鐑熻崏鎬诲叕鍙搁儜宸炵儫鑽夌爺绌堕櫌鍥藉鐑熻崏璐ㄩ噺鐩戠潱妫€楠屼腑蹇冿紝閮戝窞楂樻柊鎶€鏈骇涓氬紑鍙戝尯鏋潹琛? 鍙 450001 鍏抽敭璇嶏細鐑熻崏锛涢┈鏉ラ叞鑲硷紱娈嬬暀锛涢珮鏁堟恫鐩歌壊璋辨硶 鎽樿锛氬缓绔嬩簡钀冨彇婧跺墏銆佸浐鐩歌悆鍙栧皬鏌卞噣鍖栥€侀珮鏁堟恫鐩歌壊璋憋紙HPLC 锛夋硶妫€娴嬬儫鑽夊強鐑熻崏鍒跺搧涓┈鏉ラ叞鑲 锛圡H 锛夋畫鐣欓噺鐨勬柟娉曪紝骞堕噰鐢ㄨ鏂规硶妫€娴嬩簡23 涓儫鑽夋牱鍝佷腑鐨勯┈鏉ラ叞鑲兼畫鐣欍€傜粨鏋滆〃鏄庯細鈶犵敤4 mol/L 鐩 閰稿姞鐑洖娴佽悆鍙? h 鏁堟灉杈冨ソ锛涒憽鐢╒arian Microsorb 鈦僊V 100鈦? C18 鑹茶氨鏌憋紝浠?.1 mol/L 閱嬮吀姘存憾娑蹭负娴 鍔ㄧ浉锛岀瓑姊害娲楄劚锛屾祦閫?.7 mL/min 锛屾娴嬫尝闀?13 nm 锛?0 min 鍐匨H 鑹茶氨宄板拰鏉傝川鑹茶氨宄板彲浠ユ湁鏁堝垎 绂伙紱鈶㈣娉曠殑鍥炴敹鐜囦负97.11% 锛岀浉瀵规爣鍑嗗亸宸负7.13 锛呫€傝鏂规硶閫傚悎鐑熻崏鍙婄儫鑽夊埗鍝佷腑椹潵閰拌偧鐨勬祴瀹氥€ + 涓浘鍒嗙被鍙凤細S481 .8 鏂囩尞鏍囪瘑鐮侊細B 鏂囩珷缂栧彿锛?002-0861 锛?011 锛?2-0047-05 Determination of Maleic Hydrazide Residue in Tobacco and Tobacco Products with High PerformanceLiquidChromatography ZHANG HONG-FEI 锛孊IAN ZHAO-YANG 锛孴ANG GANG-LING 锛宎nd HU QING-YUAN China National Tobacco Quality Supervision Test Center 锛孼hengzhou 450001 锛孋hina Keywords锛歍obacco 锛汳aleic hydrazide 锛汻esidue 锛汬PLC Abstract锛欰 method for the determination of maleic hydrazide 锛圡H 锛塱n tobacco and tobacco products was established 锛宨n which MH in tobacco sample was extracted with a solvent 锛宎nd cleaned up by a solid phase extraction cartridge 锛宼hen detected with high performance liquid chromatography. The residue of MH in 23 tobacco samples was determined. The results showed that: 1锛塆ood results were obtainable when tobacco sample was extracted with 4 mol/L hydrochloric acid under heating and refluxing for 1 hour. 2 锛塗he MH peak was completely separated from impurity peaks in 20 minutes by reversed-phase HPLC with a Varian Microsorb-MV 100-5 C18 column under isocratic elution with 0.1 mol/L acetic acid as mobile phase at a flow-rate of 0.7 mL/ min. 3锛塗he recovery of MH was 97.11% with the relative standard deviation 锛圧SD 锛塷f 7.13%. Th