质谱技术分析磷酸化蛋白研究进展.pdfVIP

, 2006, 3 ( 1) : 73-76 JM ed M o l B io,l 2006, 3 ( 1) : 73-76 刘亚伟, 戴兵, 梅长林 , 200003 , , , , ; ; Q 5 1 Advances in the Analysis of Phosphorylated Protein by Mass Spec- trometry L IU Y aw e,i DA I B ing, ME I Chang lin D ivision of N ephrology, ChangzhengH osp ital, The SecondM ilitary M ed ical University, K idney D is- ease Center of PL , Shanghai, 200003, China Abstract Reversib le phosphory la ion of pro e ins is one of he m os mi por an pos- ransla ional m odif ica ions and is also invo lved in a hos of cellu lar processesA l hough full ana lysis o f phospho- ry la ed pro eins rem a ins a form idable analy ical challenge, m ass spec rom e ry has em erged as an in- creasing ly v iab le oo l for h is askTh is rev iew summ arizes he m e hodo log ies curren ly ava ilab le for he analysis of phosphory la ed pro eins by m ass spec rom e ry inc lud ing mi m unoprecipi a ing pro eins using an ibodies aga ins phosphory la ed residues, fluorescen phosphopro e in gel s a in ing, mi m obi- lized m e al affin iy chrom a ography, chem ical modifica ion, s rong ca ion exchange o enrich he phosphopro eins or phosphopep idesT andem m ass spec rom e ry approach and elec ron cap ure d is- soc ia ion have been applied for he iden if ica ion of phosphory la ion and ana lysis of phospho ry la ion si esF ina lly, iso op ic labeling are proposed fo r he he quan ifica ion o f phospho ry la ion si es Key words Phosphopro e ins; Phosphopep ides; M ass spec rom e ry , ( re- versible phosphory la ion, RP ), , 1800 [ 2] , , 2001 , ,