转移酶高的原因人DNA甲基化转移酶3B1真核表达载体的构建与鉴定_百替生物.pdfVIP

DNA 3B1 DNA 3B1 转移酶高的原因人DDNNAA甲基化转移酶33BB11真核表达载体的构 建和鉴定 【摘要】目标：建立人DNA甲基化转移酶3B1(DNMT3B1)真核表达载体pCMV-DNMT3B1。 方式：按照 中人 序列安排并分解奇同性引物 提取 Genecredit union DNMT3B1 cDNA ; HCT116细胞总RNA;哄骗PCR技术扩增出DNMT 3B1;并将扩减产物克隆到真核表达载体 pCMV-2B中。真相：PCR扩增获得人DNMT3B1的cDNA片段大小为2.6 kb;重组子哄骗限 制性内切酶实行酶切、 判决和 序列认识获得真核表达载体 。结论： PCR DNA pCMV-DNMT3B1 乐成建立了人DNMT3B1真核表达载体;为进一步研究DNMT3B1提供了条件。 “明智医学论文网”专业的医学论文辅导网站，全程代理医学论文写作宣布供职，为您 的提升开明绿色通道。挑选“明智医学论文网”，就是您明智的挑选。 联系电话 咨 询 官方网站： 电子邮箱@ 【关键词】 DNA甲基化转移酶3B1;建立;判决 Abull craptrair coolingt Objective: To construct a particulard thtowards identify the eukaryotic expression plasmid for huma particular pCMV- 2B-DNMT 3B1. Methods: Accord to the published huma particular cDNA sequence in Genecredit union; determined of primers were respectively designed a particulard synthesized. The toting RNA wwill always beoldinedd from HCT116 cell. After developing with reverse tra particularscription polymerottom chain retinquire with (RT - PCR); the product was cloned into pCMV- 2B vector. The recomcontainerould like were finpreferred friend sequenced a particulard thtowards identified by restrictive endonucleottom digestion. Results: pCMV-DNMT3B1 eukaryotic expression vectors was successfully constructed; ingso it wwhen identified by PCR; double restrictive endonucleottom digestion a particulard sequence scientific study. Conclusion: The DNMT 3B1 eukaryotic expression vectors was successfully constructed a particulard thtowards identified. Key words DNMT 3B1; Construction; Identific 谷丙转氨酶偏高原因 DNA甲基化是真核基因组修饰的一种主要方式，DNA甲基化出现于CpG二核苷酸中的胞嘧 啶上[1]。真核细胞DNA甲基化由DNA甲基化转移酶(DNMT)催化，目前浮现的DNMT有 DNMT1、DNMT2、DNMT3A、DNMT3B及DNMT3L，其中DNMT1为撑持型甲基化酶，DNMT3A 和DNMT3B为从头分解型甲基化酶，DNMT3L