10疱疹病毒-Chapter 24.ppt

Chapter 24 Outline Classification of herpesviruses Characteristics of herpesviruses Quick diagnosis of herpesviruses  Finding inclusion body Detection of viral Ag and specific Ab Detection of viral DNA 1.Serotype :HSV-1 and HSV-2 2.Pathogenicity 2. Infectious mode in B cell Proliferative infection Non-proliferative infection * * (HHV) 1.Classification (P310 Table 24-1) ?α-herpesviruses HHV-1(HSV-1) HHV-2(HSV-2) HHV-3(VZV) ? ?-herpesviruses ? ?-herpesviruses HHV-4(EBV) HHV-8 HHV-5(CMV) HHV-6 HHV-7 ? Shape and structure Glycoprotein spike Linear ds-DNA Cubic nucleocapsid Envelope 120-130nm in diameter 162 capsomeres 2.Characteristics tegument ? Cultivation ---- replicate in intranuclear of human diploid cells and produce cytopathic effect typical CPE: ? intranuclear acidophilic inclusion body ? cell fusion form multinuclear giant cells 核内嗜酸性包涵体 (except EBV) 细胞融合形成多核巨细胞 ? Pathogenicity ? transmission ? infectious mode of virus on host cells proliferative latent infection infection activate ? common infectious types (by many different routes) *subclinical infection *primary infection and latent infection *congenital infection *integrative infection 人普遍易感,感染后大多无明显症状 3.Quick diagnosis (CMV) (HSV,CMV,EBV) ? by direct contact ? by sexual contact 病人和健康带毒者 HSV-1:主要是直接密切接触, 也有性接触传播 HSV-2:性接触传播为主 ? Source of infection ? Route of transmission ? HSV-infection ? HSV-infection ? Primary infection ? Latent and recurrent infection ? Congenital and newborn infection ? Associated with cancer primary infection (anew) recurrent infection (reactivation of latent virus) (HSV-1, HSV-2) (site of latency) 复发性局部疱疹 HSV1---唇癌 HSV2---宫颈癌 大多无明显症状或出现局部疱疹 latent infection HSV-1---三叉神经节 HSV-2---骶神经节 Coldsore or fever blisters (唇疱疹或热性疱疹) ? Single serotype ? Route of transmission ? Primary infection ?Recurrent infection ? varicella (chidren) ? p