大豆异黄酮对大鼠乳腺癌细胞内cAMPPKA信号途径的影响.pdfVIP

Acta Physiologica Sinica, August 25, 2005, 57 (4): 517-522 517 cAMP/PKA * 210095 : SHZ-88 cAMP/PKA 3 50 µg/ml 15 µg/ml (RIA)cAMP (adenylate cyclase, AC)(phosphodiesterase, PDE)(γ-32P)ATP cAMP PKA RT-PCR cAMP (cAMP response element binding protein, CREB) mRNA 5 mincAMP 9.5% 11.0% (P0.05); 10 min 31.0% 40.3% (P0.01) 3 AC 5 min PDE 71.8% 71.6% (P0.05)20 minPKA 125.8% 122.3% (P 0.05); 40 min CREB mRNA 3 h 31.6% 51.1% (P 0.05); 6 h cAMP/PKA cAMP : cAMP/PKA : Q26; R737.9 Effect of soy isoflavones on cAMP/PKA pathway in breast cancer cells of the rat * LIN Cheng-Zhao, MA Hai-TianZOU Si-Xiang , WANG Guo-Jie, CHEN Wei-Hua, HAN Zheng-Kang College of Veterinary Medicine, Nanjing Agriculture University, Nanjing 210095, China Abstract: Soy isoflavones have been reported to be natural chemopreventive in several types of human cancer. Daidzein and genistein are two main components of soy isoflavones. In our previous study, they were shown to be anti-proliferative and induce cell cycle arrest at S phase of SHZ-88 rat breast cancer cells. We hypothesized that soy isoflavones might exert its anticancer effect by activating cAMP/PKA pathway. The present study was designed to analyze the effect of soy isoflavones on the cAMP/PKA pathway in SHZ- 88 cells. Daidzein and genistein were dissolved in DMSO. Cells were treated with 50 µg/ml daidzein and 15 µg/ml genistein, respectively, and with only equal DMSO in the culture medium as control. The cellular cAMP content was tested by radioimmunoassay (RIA). The 32 activity of adenylate cyclase (AC), phosphodiesterase (PDE) and PKA were measured by RIA and (γ- P) ATP incorporation. Reverse transcript-polymerase chain reaction (RT-PCR) was