栀子对铜绿假单胞菌毒力因子抑制作用及机制研究_王平.pdfVIP

中药药理与临床20 14 ;30 (6) 77 8 ， ， ， ． ． 9 ， ， ， ． ． 聂奇森 滕建文 黄丽 等 桂枝中抗过敏活性成分的研究 时珍国医 许源 宿树兰 王团结 等 桂枝的化学成分与药理活性研究进展 中 国药，2008 ;19 (7 )∶ 1594 ～ 1596 药材，20 13 ;36 (4 )∶ 674 ～ 677 Study on the antiallergic effect of different extractions of Cinnamomi ramulus Wu Zhiqiang ，He Min ，Que Changtian ，Zou Junbo ，Zhang Tong ，Yang Shengqun ，Pan Xing ，Xiong Xi ，Zeng Zhi ，Zeng Nan ＊＊ (Chengdu University of TCM ，The Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine ;State Key Laboratory Breeding Base of Systematic Ｒesearch ，Development and Utilization of Chinese Medicinal Ｒesources ，Chengdu 611137 ) Objective :To observe the antiallergic effect of nourish of different extractions of Cinnamomi Ｒamulus ，so as to explore the effective ma- terial base of its antiallergic effect and provide pharmacology basis for its efficacy and clinical application． Methods :By using the model of delayed hypersensitivity induced by DNCB in mice ，and the model of passive cutaneous anaphylaxis reaction in mice ，to preliminarily screen the effective extraction through the index of capillary permeability． Further by using the model of late hypersensitivity induced by DNCB in mice ，and the model of skin capillary permeability increasing induced by histamine or 5- serotonin in mice ，and the experiment of hyaluroni- dasee inhibition in vitro ，to validate the antiallergy effect of the above effective extractions． Ｒesults :The water decoction of Cinnamomi Ｒam- ulus without oil (6g crude drug / kg)，the extraction of ethyl acetatefractions (6g crude drug / kg)and the extraction of water (6g crude drug / kg)significantly reduced the capillary