安徽野生香菇遗传多样性及杂种优势的ISSR分析.pdfVIP

25 2211~216, 2006 Mycosystema ISSR 1 1, 2 3 1 2 3 , 230061 , 210095 , 230036 ( Intersimple sequence repeat , ISSR)1326 Lentinula edodes 6 ISSR78ISSR 6178.2%0.23325ISSR DNA Q939.5 A 1672-6472200602-0211-0216 Analysis of genetic diversity and heterosis of Lentinula edodes strains in Anhui province of China using ISSR WANG Zi-Ying1, 2 WANG Shu-Tong3 1 2 ( Department of Biology, Anhui Institute of Education , Hefei 230061; Key Lab of Monitoring and Management of Plant Disease and Insects of Chinese Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095; 3Department Of Plant Protection, Anhui Agricultural University, Hefei 230036) ABSTRACT Using technique of Intersimple sequence repeat (ISSR) 26 wild strains from Anhui province of China and 6 cultivated strains of Lentinula edodes were analyzed for genetic diversity with 13 primers. Among 78 amplified fragments 61(78.2%) were polymorphic. In 0.23 dissimilarity distance cluster analysis revealed the existence of five distinct genetic groups. The results showed that genetic similarity of these strains was low and there were obvious genetic variations in DNA level. The heterosis in the crossing combinations was measured according to the genetic distance of the parents. The results revealed that high productive hybrids were obtained by crossing parents with larger genetic distances. KEY WORDS: Dissimilarity distance, cluster analysis, crossbreeding Lentinula edodes (Berk.) Pegler L. edodes ( ). Email: sjywzy@ 2005-09-142005-12-12 212