孤束核在针刺“足三里”调节胃运动中的作用机制_临床医学论文.docVIP

孤束核在针刺“足三里”调节胃运动中的作用机制_临床医学论文 孤束核在针刺“足三里”调节胃运动中的作用机制_临床医学论文 作者：王述菊,孙国杰,杜艳军,胡和平,吴绪平,孔立红 【摘要】 目的 探讨迷走神经中枢孤束核在针刺“足三里”对胃运动障碍大鼠胃内压影响中的作用。方法 采用神经电生理学方法,用微电极细胞外记录大鼠孤束核单个神经元的活动,在正常生理状态下及经血管注射抑制胃运动的药物阿托品或促进胃运动的药物胃复安后,观察针刺“足三里”对孤束核神经元放电及胃内压的影响。结果 在正常生理状态下,针刺“足三里”有效激活了孤束核神经元放电,并明显促进胃运动。静脉注射阿托品后,孤束核神经元背景放电减少,但针刺对放电的影响与药物前相比变化不大, 静脉注射阿托品后针刺“足三里”仍有效激活了孤束核神经元放电,且静脉注射阿托品后针刺“足三里”能明显促进胃运动。在静脉注射胃复安后,孤束核神经元背景放电增加,但针刺对放电的影响与药物前相比,变化也不大, 静脉注射胃复安后针刺“足三里”仍有效激活了孤束核神经元放电,而且静脉注射胃复安后针刺“足三里”更明显促进胃运动。结论 针刺“足三里”对胃功能的调节作用以促进胃运动为主,其调节作用与孤束核神经元的激活密切相关,且针刺“足三里”引起孤束核相关神经元放电增加幅度不受周围胆碱能受体阻滞剂阿托品及多巴胺受体拮抗剂胃复安的影响。 【关键词】 孤束核；足三里；针刺；胃运动；大鼠 Abstract：Objective To research the function of the vagus nerve center nucleus of solitary tract (NTS) in the influence of gastric pressure by acupuncture at “Zusanli” in rats with motility disturbances of the stomach. Methods Adopting electrophysiological methods, the extracellular discharge signals of neuronic action in NTS were recorded with glass microelectrode. The influence of the discharges of NTS neurons and gastric pressure responding to the acupuncture at “Zusanli” point were observed in normal status and the condition of Atropine or Metoclopramide intravenous injection. Results In the normal status, acupuncture at “Zusanli” activated the discharges of NTS neurons and promote the gastric pressure remarkably. After Atropine intravenous injection, the background discharges of NTS neurons decreased. There was no statistical difference in the discharges responding to the effect of acupuncture before and after Atropine administration. And the gastric motion reinforced obviously by acupuncture at “Zusanli” after Atropine administration. But after intravenous injection of Metoclopramide, the background discharges of NTS neurons increased. There was also no statistical difference in the discharges responding to the effect of acupuncture before and after Metoclopramide administration. Moreover, the function of promoting gastric motion by acupuncture at “Zusanli” got more