糖尿病牙周病大鼠牙周龈沟液中TNF-α, IL-1β 和 LPS 水平的研究.docVIP

  糖尿病牙周病大鼠牙周龈沟液中 TNF-α, IL-1β 和 LPS 水平的研究# 姜竹玲1，李莹2，王祥2，张斌1** 5 10 15 20 25 30 35 40 （1. 哈尔滨医科大学附属第二医院口腔种植科； 2. 哈尔滨医科大学附属二院硬组织发育与矿化研究所） 摘要：目的.本文主要研究了糖尿病牙周病大鼠模型龈沟液中 TNF-α, IL-1β和 LPS 水平的 改变。方法.15 只大鼠通过注射链脲佐菌素建成糖尿病模型并使用丝线诱导成牙周病模型 （组 1），15 只大鼠丝线诱导成牙周病模型（组 2），15 只大鼠链脲佐菌素注射建成糖尿病 模型（组 3），15 只系统健康大鼠作为正常对照组（组 4）。用 ELISA 方法检测龈沟液中 TNF- α, IL-1β和 LPS 水平。结果.组 1 与组 2、3、4 相比 TNF-α, IL-1β和 LPS 水平较高（p0.05）， 组 2 中的水平比组 3、4 高（p0.05），组 3 的比组 4 的水平高（p0.05）。组间数据有统 计学意义。结论.数据表明糖尿病可以提高牙周龈沟液中 TNF-α, IL-1β和 LPS 的水平。 关键词：肿瘤坏死因子-α；白介素-1β；脂多糖；糖尿病；牙周病 中图分类号：R781 Study of TNF-α, IL-1β and LPS Levels in the Gingival Crevicular Fluid of a Rat Model of Diabetes Mellitus and Periodontitis JIANG Zhuling1, LI Ying2, WANG Xiang2, ZHANG Bin1 (1. Department of implantology, the 2nd Affiliated Hospital, Harbin Medical University; 2. Institute of hard tissue development and regeneration, the 2nd Affiliated Hospital, Harbin Medical University) Abstract: Objective. In this study, we sought to investigate the dynamic changes in the levels of TNF-α, IL-1β and LPS in the GCF in a rat model of diabetes mellitus (DM) and periodontitis (PD). Methods. DM and PD were induced together in 15 rats (group 1) by streptozotocin injection and ligature induction. Periodontitis alone was produced by ligature induction in 15 rats (group 2), diabetes alone was produced by streptozotocin injection in 15 rats (group 3), and fifteen systemically and periodontally healthy rats were used as controls (group 4). The gingival TNF-α, IL-1β and LPS levels were measured by using ELISA method. Results. Higher levels of TNF-α, IL-1β and LPS were found in group 1 compared with group 2, group 3 and group 4 (p0.05). The quantities of TNF-α, IL-1β and LPS were greater in group 2 than group 3 and group 4 (p0.05). Group 3 demonstrated higher levels of TNF-α, IL-1β and LPS than group 4 (p0.05). Statistically significant differences were noted between all of the groups. Conclusions. These data indicate that DM may lead to enhanced TNF-α, IL-1