纳米级控制丝蛋白用于构建多孔支架.docVIP

  Nanoscale Control of Silks for Porous Scaffold Formation# Lin Shasha1, Zuo Baoqi1, Lv Qiang1, Zhu Hesun2** 5 10 15 20 25 30 35 40 45 (1. National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou 215123, People’s Republic of China; 2. Research Center of Materials Science, Beijing Institute of Technology, Beijing, 100081, People’s Republic of China) Abstract: Silk-based porous scaffolds have been used extensively in tissue engineering because of their excellent biocompatibility, tunable biodegradability and robust mechanical properties. Although many silk-based scaffolds have been prepared through freeze-drying, a challenge remains to effectively control the porous structures during this process. In the present study silk fibroin with different nanostructures were self-assembled in aqueous solution and then used to regulate the porous structure of freeze-dried scaffolds. Viscosity, secondary structure and water interactions were also studied to elucidate their influence on the formation and control of porous structures. A mechanism of control of such features is provided based on preformed nanostructures from the silk fibroin as a critical factor to regulate the final features of these porous structures. Once silk self-assembled into nanofilaments in aqueous solution, porous structures were easily formed in the freeze-drying process. These soluble porous scaffolds were then treated with water- or methanol-annealing to achieve water-stability and different degradation behaviors. This nanoscale control offers detailed control of the matrix features which are important in regulating cell and tissue outcomes. Key words: Tissue engineering; Silk; Scaffolds; Nanostructure; porous structure 0 Introduction Silk-based materials have been transformed in just the past decade from the commodity textile world to a growing web of applications in tissue engineering due to the impressive biocompatibility, biodegrad