藏山羊脂联素基因的克隆与表达分析.docVIP

  藏山羊脂联素基因的克隆与表达分析# 薛科，李雪梅，仲涛，李利，张红平，王林杰** （动物遗传育种研究所，四川农业大学动物科技学院，成都 611130） 5 10 15 20 25 30 摘要：本研究旨在克隆藏山羊脂联素（AdipoQ）基因，并且分析其组织表达模式。利用生 物信息学和比较基因组学方法克隆得到藏山羊 AdipoQ 基因序列。半定量 RT-PCR 检测 AdipoQ 基因在成年藏山羊 11 个不同组织中的表达模式；QRT-PCR 检测成年藏山羊不同部 位脂肪组织中 AdipoQ 基因的表达量差异。结果表明：藏山羊 AdipoQ 基因编码区与其它哺 乳动物 AdipoQ 基因同源性较高，同时脂联素球状蛋白结构域高度保守。AdipoQ 基因在皮 下脂肪中表达量最高，在肌肉组织和胃中表达量较高，而在其他各组织未检测到基因表达。 QRT-PCR 发现 AdipoQ 在肾周脂肪组织中的表达量高于肠系膜脂肪组织和皮下脂肪组织中 的表达量，但表达差异不显著。本研究得到藏山羊 AdipoQ 基因序列及组织表达规律，为进 一步研究藏山羊脂联素基因的功能作奠定基础。 关键词：脂联素；藏山羊；脂肪沉积 中图分类号：S827 Cloning and expression analysis of AdipoQ gene in Tibetan goat Xueke, Li Xuemei, Zhong Tao, Li Li, Zhang Hongping, Wang Linjie (Institute of Animal Genetics and Breeding, College of Animal Science and Technology,Sichuan Agricultural University,Chengdu, 611130) Abstract: In this paper，we identify the AdipoQ gene, and analyse its expression pattern in Tibetan goat. We used the bioinformatics and comparative genomics methodology to clone the Tibetan AdipoQ gene. Semi-quantative RT-PCR was performed to analyse the mRNA expression profile of AdipoQ gene in eleven tissues of Tibetan goat. QRT-PCR was used to detect the expression pattern of AdipoQ in three different adipose tissues of Tibetan goat. The result showed that the AdipoQ gene in Tibetan goat had high identity with those in other mammals, and the globular domain is a conserved region among various species. We found that AdipoQ were highly expressed in adipose tissue and skeletal muscle. The expression of AdipoQ in perirenal fat tissue was higher than those in subcutaneous fat tissue and mesenteric fat tissue, but the expression difference was not significant. In this study, we obtained the sequence and expression pattern of AdipoQ gene which laid a foundation for further studying its function in Tibetan goat. Key words: AdipoQ；tibetan goat；fat deposition 35 0 引言 脂联素是一种脂肪细胞因子，由四个独立的研究机构通过不同的方法发现并命名 Acrop30[1]，AdipoQ[2]，qpM1[3]，GBP28[4]。最初认为脂联素蛋白只在脂肪组织中表达；但后 续研究发现，脂联素在骨骼肌、大脑、肝脏、血管内皮等组织中也有表达[5-7]。脂联素前体