药用甘草组织培养生产黄酮的研究进展.pdfVIP

植物生理学报 Plant Physiology Journal 2013, 49 (1): 13~ 18 13 药用甘草组织培养生产黄酮的研究进展 * , , 焦艳红 宋艳茹 高述民 , 100083 北京林业大学生物科学与技术学院 北京 : “ ” 摘要 甘草是驰名中外的中药材, 有 十方九草 之称, 甘草黄酮为其主要成分之一, 不仅具有消炎保肝、抗氧化、抗肿瘤、 抗病毒和抗菌等药用功效, 还可用于食品、化妆品等工业生产中。本文介绍了甘草黄酮的种类、组织化学定位, 并着重总 结了黄酮的组织培养获得途径及生物合成调控的影响因素, 在此基础上, 对今后的研究及应用进行了分析和展望, 旨在为 更好地开发利用甘草黄酮提供一定的依据。 : 关键词 甘草; 甘草黄酮; 细胞培养; 毛状根; 不定根 Research Advances on Production of Flavonoids by Licorice Tissue Culture * JIAO Yan-Hong, SONG Yan-Ru, GAO Shu-Min College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China Abstract: Licorice is an important component in many Chinese traditional medicines. As its secondary metabo- lites, licorice flavonoids not only have antiinflammatory, antioxidative, antitumor, antiviral and antibacterial ef- fects, but also are used in food, cosmetics and other industries. Here we briefly described the classification and histochemical localization of licorice flavonoids, the recent progresses in the production of licorice flavonoids by different tissue culture approaches the regulation of influence factors among biosynthesis were summarized simultaneously. Finally, the problems remained to be studied and the possible research directions in this field are proposed. It can support a new insight into further study on utilization of licorice flavonoids. Key words: licorice; Glycyrrhiza flavonoids; cell culture; hairy root; adventitious root 甘草为豆科(Leguminosae)甘草属(Glycyrrhi- 1 甘草黄酮类化合物及价值 za) , , (4%~24%) 的多年生草本植物 主要以根茎入药 具补脾益 甘草活性成分主要为三萜皂苷类 气、清热解毒、润肺止咳、缓急定痛、调和诸药 和黄酮类(1%~5%)及少量的多糖、生物碱、木