基于层层自组装安培免疫传感器的研究.docVIP

 基于层层自组装安培免疫传感器的研究# 高丽娟** （宁波大学材料科学与化学工程学院，宁波 315211） 5 10 15 20 25 30 35 40 摘要：该文基于层层组装技术，构建了一种无媒介安培免疫传感器用于动物源食品中瘦肉精 残留测定。采用天然的铁卟啉（hemin）作为电子媒介体，利用静电吸引将其组装在玻碳电 极表面，并将 CLB 抗体（anti-CLB）固定于最终修饰完整的电极表面，合成了兼具电活性 的{hemin /PAH-Au}n 的纳米探针。最后将修饰了 CLB 抗体的电极浸泡在一定浓度的 CLB 抗原中，由于抗原会阻碍电子的传递，所以当越来越多的抗原结合到电极上时，会引起电流 信号的的越来越弱，该文中就是根据这样的电流下降值来进行定量分析，建立基于铁卟啉修 饰电极的一步免疫免疫检测方法。检测结果表明，在优化条件下，该法检测 CLB 的检测限 为 0.02 ng/mL，检测范围为 0.04-1.2 ng/mL。该实验中，层层组装用于电极修饰，非常方便 且快速，最重要的是层层组装很好的提高了电信号，加大了检测范围，还有于增加传感器稳 定性，该免疫传感器具有成本低廉、灵敏度高、无标记、寿命长、可大规模生产，对测定样 品无污染等优点，可实现现场和在线检测。 关键词：层层自组装；铁卟啉；聚丙胺基盐酸盐；瘦肉精； A novel amperometric immunosensor based on layer-by-layer self-assembly technology GAO Lijuan (Ningbo University, Faculty of Material Science and Chemical Engineering, Ningbo 315211) Abstract: In this paper, a non-media amperometric immunosensor for the detection of clenbuterol is constructed based on layer-by-layer assembly technique. Using the natural iron porphyrin (hemin) as an electron mediator,and it is modified on the glassy carbon electrode surface by electrostatic attraction,then CLB antibodies (anti-CLB) is also fixed to the final completely modified electrode surface,then the nano-probe is prepared with {hemin / PAH-Au}n which both have the electrical activity .Finally,the electrode modified by CLB antibody was immersed in a certain concentration of CLB antigen,.However, the CLB antigen will hinder the transfer of electron.So when a growing number of antigen binding to the electrodes, the current signal will be weaker,.This paper is based on this current decline to make quantitative analysis,to establish an immunization immunoassay based on iron porphyrin modified sensor. Test results show that, Under optimal conditions, the assay detection limit of CLB is 0.02 ng / mL, and the detection range is 0.04-1.2 ng / mL. In this experiment, the sensor is modified by the layer-by-layer technology which is very convenient and fast, What’s more, the layer-by-layer technology can well increase the s