基于线粒体控制区序列探讨大头乌龟物种的有效性.docVIP

 基于线粒体控制区序列探讨大头乌龟物种 的有效性# 黄振锋，聂刘旺，夏行权，刘罗，景万兴，卜兴江，刘娟娟** 5 10 15 20 25 30 35 40 （安徽师范大学生命科学学院，安徽重要生物资源保护与利用研究省级重点实验室，安徽芜 湖 241000） 摘要：大头乌龟(Chinemys megalocephala)物种的有效性一直存在争议，尚没有得到一致的观 点。本文采用特异引物，扩增、测序获得了 2 只大头乌龟（2♀）、7 只乌龟（Chinemys reevesii ） (4♀, 3♂)的线粒体控制区序列。在获得的 9 个个体线粒体控制区中，都识别了 3 个区域： TAS 区，CD 区和 CSB。其中 CD 区含有一个保守序列框 F(CSB-F)，CSB 区含有 3 个保守 序列框(CSB-1, CSB-2, CSB-3)。另外，对 CSB 区 3’端的可变数目的串联重复（VNTRs） 或微卫星序列分析发现，大头乌龟个体的基序类型以及基序的排列顺序与乌龟一致。基于控 制区 811 bp 的序列，分别用最大简约法(MP)和贝叶斯法(BI)重建了系统发育树，探讨了大头 乌龟与乌龟的种间关系。系统发育树显示大头乌龟并没有形成一个独立的进化枝，其在系统 进化树上嵌套在乌龟物种中。基于上述结果，我们不支持大头乌龟是一个有效物种。 关键词：大头乌龟；乌龟；线粒体控制区；可变数目的串联重复 中图分类号：Q959.6; Q951.3; The Validity Research of the Species Chinemys megalocephala Based on Sequences of Control Regions of Mitochondrial DNA HUANG zhenfeng, NIE liuwang, XIA xingquan, LIU luo, JING wanxing, BU xingjiang, LIU juanjuan (Life Science College, Anhui Normal University,The Provincial Key Lab of the Conservation and Exploitation Research of Biological Resources in Anhui, Wuhu, Anhui 241000, China) Abstract: The validity of the species Chinemys megalocephala has been under debate for years. However, there hasn’t been a consistent view on it. In this study, the mtDNA control regions for 2 Chinemys megalocephala (♀) and 7 Chinemys reevesii (4♀, 3♂) were amplified and sequenced using specific primers. Three domains (TAS domain, CD domain, CSB domain) were identified and compared among control regions of the two individuals of Chinemys megalocephala and seven individuals of Chinemys reevesii. It showed that the CD domain had a conserved sequence block F (CSB-F) and the CSB domain had three conservative sequence blocks (CSB-1, CSB-2, CSB-3) for the above nine individuals. In addition, the analysis of variable number tandem repeats (VNTRs) of 3’ end of CR showed that the motif types of tandem repeat and the arrange order of motifs in Chinemys megalocephala were almost the same with Chinemys reevesii. Based on 811 bp sequences of control regions, the phylogenetic trees