顺铂-羟基磷灰石-壳聚糖缓释微球的合成及其性状研究.docVIP

 顺铂-羟基磷灰石-壳聚糖缓释微球的合成及 其性状研究# 冯卫1，付莉2，刘建国1** 5 10 15 （1. 吉林大学第一医院，长春市，130021； 2. 吉林大学第二医院，长春市，130021） 摘要：目的 制备顺铂-纳米羟基磷灰石-壳聚糖（DDP-HAP-CS）缓释微球，观察其性状和载 药率。方法 以纳米羟基磷灰石（HAP）、壳聚糖（CS）和顺铂（DDP）为原料，采用乳化 交联法制备 DDP-HAP-CS 缓释微球；采用扫描电子显微镜观察微球的大小和表面形貌，X 射线衍射仪分析交联后微球的物相及其结晶程度；原子吸收分光光度计测定缓释微球中 DDP 的含量，动态渗析法检测该缓释微球 DDP 体外释放曲线。结果 成功制备了 DDP-HAP-CS 缓释微球，直径约为 5μm-20μm，微球中主要为弱结晶 CS 和 HAP 晶相。微 球中 DDP 的含量为 16.46%，该缓释微球 DDP 体外释放释放率随时间延长而增加，24h 内 DDP 的释放率增加较快，24h-72h 释放率处于平台期，72h 后释放率又开始缓慢上升，至 120h 后重新进入平台期。结论 DDP-HAP-CS 缓释微球可以使 DDP 在体外条件下缓慢释放，基 本达到了缓释药物的要求。 关键词：外科学；顺铂；纳米；羟基磷灰石；壳聚糖 中图分类号：R738.1 20 Experimental Study of Synthesis and Characteristics of Cisplantin- nano Hydroxyapatite-Chitosan Microspheres Feng Wei1, Fu Li2, Liu Jianguo1 (1. the First Hospital of Jilin University,Changchun, 130021; 25 30 35 2. the Second Hospital of Jilin University,Changchun,130021) Abstract: IObjective To prepare Cisplantin-nano Hydroxyapatite-Chitosan Microspheres, and investigate its characteristics. Methods Nano-hydroxyapatite (HAP), chitosan (CS) and cisplatin (DDP) were as raw materials, and DDP-HAP-CS release microspheres were prepared by emulsion cross-linked method. The size and surface morphology of microspheres were observed by scanning electron microscopy, and the phase and crystallinity of microspheres were detected by X-ray diffraction analysis. DDP content of microspheres was detected by atomic absorption spectrophotometer, and the DDP release profile of microspheres was detected by dynamic dialysis method. Results DDP-HAP-CS release microspheres were prepared with diameter of 5μm-20μm, and DDP content of microspheres was 16.46%. The release rate of DDP from microspheres increased with time in vitro. The release rate of DDP increased rapidly in the first 24h, and the release rate reached plateau stage in 24-72h, then the release rate rose slowly, and reached plateau stage again after 120h. Conclusion DDP-HAP-CS release microspheres can make DDP slow release in vitro, it can meet the bas