铁离子对餐厨垃圾糖化液发酵产乳酸的促进作用分析.docVIP

 铁离子对餐厨垃圾糖化液发酵产乳酸的促 进作用分析# * 5 10  摘要：为了验证铁离子的加入能提高餐厨垃圾发酵乳酸产量，本试验组考察在餐厨垃圾糖化 液中添加铁离子及未添加时乳酸的发酵情况。结果表明未添加铁离子条件下葡萄糖消耗速率 较慢，在发酵 72h 后，其含量为 4.72g/L，乳酸的最高产量为 17.8g/L；添加铁离子时，葡萄 糖含量下降速度较快，发酵 60h 后浓度约为 1.97g/L，乳酸产量为 27.1g/L。对酶活的研究发 现，添加铁离子条件下 LDH 酶活性为 11.31 U/mg，较未添加的酶活性提高 43.3%，ADH 活 性的变化的趋势刚好与 LDH 的相反，这与乙醇含量变化规律相符。通过对代谢过程中各个 指标的测定，验证了铁离子的加入可以提高餐厨垃圾产乳酸的产量。 关键词：环境科学与工程；铁离子；餐厨垃圾；乳酸；发酵 中图分类号：X705 15 Stimulative effect of ferricion on kitchen garbage saccharification liquid fermentation to lactic acid XIANG juan, LIU jianguo, ZOU Hui, Wang Qunhui (School of Civil and Environment Engineering, University of Science and Technology Beijing, 20 25 30 35 40 Beijing 100083) Abstract: In order to verify the addition of ferricion can improve kitchen waste fermentation production of lactic acid, we examined the fermentation condition of lactic acid by adding ferricion and not adding in the kitchen garbage saccharification liquid. The results show that glucose consumption rate is slower under the condition of not adding ferricion. After 72h fermentation, glucose content was 4.72g/L, the maximum output of lactic acid 17.8g/L .However, glucose content decreased faster by adding ferricion, and the concentration of glucose was about 1.97g/L, lactic acid yield 27.1g/L after 60h. Enzyme activity study found enzyme activity of LDH is 11.31 U/mg under the condition of adding ferricion, increasing by 43.3%than which didn’t. Changes of the ADH and LDH activity have opposite trends, which matches the content change rule of ethanol. Based on the determination of various indexes in the metabolic processes, it verify the addition of ferricioncan improve the kitchen waste to produce lactic acid yield. Keywords: Environmental Science and Engineering; ferricion; kitchen waste; lactic acid; fermentation 0 引言 嗜淀粉乳杆菌是一种具有淀粉水解活性的乳酸细菌，它产生的解淀粉酶具有淀粉酶和支 链淀粉酶双重活性，可以直接转化复杂的淀粉质底物生成乳酸[1]。餐厨垃圾是一种生物质废 弃物，有机物含量高，且以淀粉类物质为主要成分[2]。利用嗜淀粉乳杆菌生产市场需求量巨 大的乳酸[3]，是餐厨垃圾资源化利用的重要研究方向。 本试验组已经利用 C13同位素示踪法研究了合成培养基中嗜淀粉乳酸菌的糖代谢通量分