文冠果壳苷对正常小鼠胆碱能系统的作用.docVIP

 文冠果壳苷对正常小鼠胆碱能系统的作用# 5 10 15 20 25 30 35  摘要：前期研究发现文冠果壳苷能显著改善痴呆模型小鼠的学习记忆障碍，但仍不明确其作 用机制是否与影响中枢胆碱能系统有关，因此本研究考察文冠果壳苷对正常小鼠中枢胆碱能 神经系统的影响。选用昆明种小鼠，连续灌胃给予文冠果壳苷 8 天或 14 天，用酶联免疫分 析（ELISA）方法检测了文冠果壳苷对正常小鼠大脑海马组织内胆碱乙酰转移酶（ChAT） 的含量及活性、乙酰胆碱（ACh）的含量、乙酰胆碱酯酶（AChE）含量及活性的影响；用 反转录-聚合酶链式反应（RT-PCR）的方法检测了文冠果壳苷对正常小鼠大脑海马组织内 ChAT mRNA 的表达水平的影响。结果表明文冠果壳苷对正常小鼠脑组织海马内 AChE 活性 及含量均未见显著增强作用，但可以剂量依赖性地增加正常小鼠海马组织内 ACh 的含量， 增强 ChAT 的含量及活性，并提高正常小鼠海马组织内 ChAT mRNA 的表达水平。以上结 果提示文冠果壳苷可能通过改善中枢胆碱能系统的功能而发挥健脑促智的效果。 关键词：药理学；文冠果壳苷；阿尔茨海默病；中枢胆碱能系统 中图分类号：R96 Effect of xanthoceraside on central cholinergic system in normal mice ZOU Libo1, WANG Lihua2, CHI Tianyan1, JI Xuefei1, WANG Haiping1 (1. Department of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang 110016; 2. Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016) Abstract: Our previous study demonstrated that xanthoceraside had protective effects on learning and memory impairments of Alzheimers –model mice, but whether its mechanisms is related to central cholinergic system remained unclear. The present study is to investigate the effect of xanthoceraside on central cholinergic system in normal mice. We orally administered xanthoceraside to male kunming species mice for 8 days or 14 days, then the content and / or activity of choline acetyltransferase (ChAT), acetylcholinesterase (AChE) and acetylcholine (ACh) in hippocampus of mice were detected by ELISA assay. The level of ChAT mRNA expression in hippocampus of mice was determined by RT–PCR assay. The results showed that the administration of xanthoceraside significantly increased the content of ChAT and ACh and enhanced the activity of ChAT, but has no markly effect on the content as well as the activity of AChE. Meanwhile, xanthoceraside could significantly increase the expression level of ChAT mRNA in hippocampus of mice. These data suggest that xanthoceraside may improve learning and memory impairments through the improvement of central cholinergi