大黄酸对内毒素激活小胶质细胞的影响.docVIP

 大黄酸对内毒素激活小胶质细胞的影响# 景浩然，柴丽娟，郭虹，李美娇，刘青青，胡利民，王少峡** （天津中医药大学中医药研究院，天津市中药药理学重点实验室，教育部方剂学重点实验室， 5 10 15 20 25 30 35 40 天津 300193） 摘要：目的：抑制小胶质细胞的过度活化,对防治中风、神经退行性疾病有重要意义。本研 究旨在观察大黄酸对小胶质细胞细胞株的作用。方法：培养小胶质细胞细胞株，检测大黄酸 对小胶质细胞活力及内毒素（LPS）诱导小胶质细胞一氧化氮（NO）产生的影响。结果：大 黄酸可以在不影响细胞活力的情况下抑制 NO 在激活小胶质细胞中的产生。结论：大黄酸可 以抑制 LPS 对小胶质细胞的激活。 关键词：大黄酸；小胶质细胞；炎性反应 中图分类号：R9 Effects of 1.8-Dihydroxy-3-carboxy-anthraquinone on LPS-induced microglial activation JING Haoran, CHAI Lijuan, GUO Hong, LI Meijiao, LIU Qingqing, HU Limin, WANG Shaoxia (Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin Key Laboratory of Chinese medicine Pharmacology, Tianjin University of Traditional Chinese Medicine, TianJin 300193.) Abstract: Objective: Inhibition of the microglial over-reaction is useful for alleviating the progression of neurological diseases, including stroke and neurodegenerative diseases. This study examined whether 1.8-Dihydroxy-3-carboxy-anthraquinone, a metabolite from Salvia miltiorrhiza, may exert an anti-inflammatory effect in microglia.Methods: We examined that the effect of 1.8-Dihydroxy-3-carboxy-anthraquinone on cell viability and the production of nitric oxide (NO) induced by LPS treatment in rat primary cultured microglia.Results: 1.8-Dihydroxy-3-carboxy -anthraquinone could significantly inhibit the production of NO by LPS treatment in rat primary cultured microglia.Conclusion: 1.8-Dihydroxy-3-carboxy-anthraquinone could suppress microglial activation and this might be a mechanism underlying the neuroprotective activity of it. Keywords: 1.8-Dihydroxy-3-carboxy-anthraquinone; microglia; inflammation 0 引言 小胶质细胞（microglia）是中枢神经系统免疫细胞，参与脑缺血、感染、变性等急慢性 炎性损伤的病理生理过程，在中风、神经退行性疾病、AIDS 引起的痴呆等疾病发展过程中 都发挥重要作用。小胶质细胞的活化虽对 CNS 具有一定的保护作用,但过度活化则可产生各 种促炎因子从而介导神经元死亡[1]。因此，抑制小胶质细胞的过度活化,平衡其分泌的各种促 炎因子,是防治中风、神经退行性疾病的重要途径[2]。新近实验证实，四环素[3]、纳洛酮[4]及 传统中药成分穿心莲内酯[5]、雷公藤内酯醇[6]等可以通过抑制小胶质细胞的炎性反应起到神 经保护作用。 大黄具有抗炎、抗肿瘤、恢复肾功能、保肝利胆等作用，它在治疗预防和治疗多器官功 能不全综合征、全身性炎症反应综合征和多器官功能紊