MicroRNA let-7i转染诱导的CD86-DCs对Treg作用的研究.docVIP

 MicroRNA let-7i 转染诱导的 CD86-DCs 对 Treg 作用的研究# 吴健1,2，张毛毛1,2，刘芳1,2，韩晓丽1,2，谭妙欣1,2* 5 10 15 20 25 30 35 40 45 （1. 哈尔滨医科大学，心肌缺血机理与诊疗技术教育部共建重点实验室，哈尔滨 150081； 2. 哈尔滨医科大学附属第二医院心血管内科，哈尔滨 150081） 摘要：目的进一步研究 miRNAlet-7i 的免疫调节作用是通过 DC 的何种亚群发挥作用的，以 及此亚群对 Treg 的影响。方法用免疫磁珠将 miRNAlet-7i 抑制剂处理的 DCs 分成两组： + - + - 对 Treg 数量的影响，对上清液 IL-10 分泌的影响；Western blotting 检测 miRNAlet-7i 的 靶点 SOCS1 在不同细胞亚群的表达。结果转染 miRNAlet-7i 抑制剂明显降低了 LPS 刺激的 + - - 诱导 T 细胞的低反应性和抗炎症细胞因子的分泌,并且抑制促炎症细胞因子的分泌，从 - + + - - 关键词：树突状细胞；miRNAlet-7i；调节性 T 细胞；细胞因子 中图分类号：R392.4；R392.1 The role of miRNA let-7i transfection-induced CD86-DC on Treg action WU Jian1,2, Zhang Maomao1,2, Liu Fang1,2, Han Xiaoli1,2, Tan Miaoxin1,2 (1. Key Laboratories of Education Ministry for Myocardial Ischemia Mechanismand Treatment, Harbin Medical University, Harbin 150081; 2. Department of Cardiology, Second Af?liated Hospital of Harbin Medical University, Harbin 150081) Abstract: Objective Too further research through which DC subsets did miRNAlet-7i play a role in immune regulation and the impact of this sub-group on Treg. Methods miRNAlet-7i inhibitor transfected DC were divided into CD86+DCs and CD86-DCs using immunomagnetic beads, cocultured with T cells and observed the effect of CD86+DC and CD86-DC on T cell proliferation、the number of Treg and IL-10 secretion. The expression of SOCS1 of different cell subsets which is the target of miRNAlet-7i is detected by Western blotting. Results Transfecting with miRNAlet-7i inhibitor significantly impeded DC surface expression of CD80 and CD86 and the secretion of pro-inflammatory cytokine. There were two subpopulations of LPS-stimulated DCs with downregulated miRNAlet-7i, CD86+DC and CD86-DC, and it was the CD86-DCs that were more effective in inducing T cell hyporesponsiveness and the secretion of anti-inflammatory cytokine,and inhibiting secretion of pro-inflammatory cytokine.CD4+CD25+Tregs, isolated from CD86-DCs