环孢素A通过调节母-胎界面CXCL12和CD82的表达来提升滋养细胞侵袭力.docVIP

 n 环孢素 A 通过调节母-胎界面 CXCL12 和 CD82 的表达来提升滋养细胞侵袭力# 李明清，孟玉菡，邵珺，李辉，李大金** 5 10 15 20 25 30 （复旦大学附属妇产科医院，上海 200011） 摘要：目的：为了探讨环孢素 A (CsA)是否可以通过调节人早孕期母-胎界面 CXCL12 和 CD82 的表达来调节滋养细胞侵袭力。方法：in-cell Western 检测 CsA 是否直接或间接通过训导滋 养细胞功能改变来调节蜕膜基质细胞（DSCs）CD82 表达；ELISA 检测 CsA 是否调节 DSC 和或滋养细胞 CXCL12 的分泌；in-cell Western 检测 CsA 是否通过调节滋养细胞 CXCL12 的分泌水平来调节 DSCs 的 CD82 表达；Matrigel 侵袭实验分析 CsA 是否调节母-胎界面 CXCL12 和 CD82 表达来进一步调控滋养细胞的侵袭力。结果：CsA 促进滋养细胞分泌 CXCL12。CXCL12 一方面以自分泌的方式促进滋养细胞的侵袭力；另一方面，通过上调 DSCs 的 CD82 表达来控制滋养细胞的侵袭力。结论：CsA 通过调节人早孕期母-胎界面 CXCL12 和 CD82 的表达，加强滋养细胞和 DSCs 间交互对话来促进滋养细胞侵袭。 关键词：环孢素 A；母胎界面；CXCL12；CD82；滋养细胞；侵袭力 中图分类号：R71 CsA improves trophoblasts invasiveness by regulating the expression of CXCL12 and CD82 at maternal-fetal interface LI Mingqing, MENG Yuhan, SHAO Jun, LI Hui, LI Dajin (Gynecology and Obstetrics Hospital of Fudan University, Shanghai 200011) Abstract: Objective: To explore whether cyclosporine A (CsA) can modulate trophoblasts invasiveness through regulating the expression of CXCL12 and CD82 at maternal-fetal interface in early pregnancy. Methods: In-cell western was used to analyze whether CsA can regulate the expression of CD82 through educating the function of trophoblasts; ELISA was used to delete the secretion of CXCL12 of DSCs and or trophoblasts; then we evaluated the effect of CsA on the trophoblasts invasiveness by Matrigel invasion assay. Results: CsA can stimulate the CXCL12 production of trophoblast. On the one hand, CXCL12 increases the trophoblasts invasiveness in an atuocrine manner; on the other hand, it also controls the invasiveness of trophoblasts by up-regulating the expression of CD82. Conclusion: CsA may strengthen the cross-talk of trophoblasts and DSCs and further increase trophoblasts invasiveness via regulating the expression of CXCL12 and CD82 at the maternal-fetal interface. Key words: cyclosporin A; maternal-fetal interface; CXCL12; CD82; trophoblasts; invasiveness 35 0 引言 本课题组已经研究证实，低剂量 CsA 对正常妊娠模型及妊娠失败模型的存活胎鼠及其 胎盘生长无抑制作用，相反对妊娠失败模