SRAP、TRAP、SSR、ISSR分子标记在水稻籼粳分型中的系统学研究.docVIP

 SRAP、TRAP、SSR、ISSR 分子标记在水 稻籼粳分 型中的系统学研究 5 10 15 # 摘要：利用 SRAP、TRAP 新型分子标记，并结合 SSR、ISSR 标记对水稻 47 个材料进行了 遗传多样性分析，四种分子标记多态性 ISSRSRAPSSRTRAP，SRAP 标记的平均遗传相 似性为 0.70, TRAP 标记 0.74,SSR 标记 0.75,ISSR 标记 0.85；四种分子标记之间都呈极显著 相关；聚类分析中籼粳稻都有较好的区分，在部分材料归类上有部分差异，但 SRAP 和 TRAP 较 SSR 和 ISSR 准确，而合并四种分子标记并聚类分析得到的树状图更能反映真正的 系统演化关系。实验结果表明 SRAP 和 TRAP 标记在水稻研究都有较好的利用价值，SRAP 标记在区分亲缘关系较近的品种间有更强的分辨能力。四种分子标记中爪哇稻 Lemont,POO2 都归为籼类群,说明它们与籼稻更近的亲缘关系。而普通野生稻材料在不同分 子标记的树状图中，部分材料在籼粳归类上存在差异，在合并四种标记聚类分析中，所有 野生稻材料都归为粳稻一类群，表明中国普通稻仍以偏粳为主。 关键词：水稻；SSR；ISSR；SRAP；TRAP；聚类分析 中图分类号：S332.2 20 Molecular Phylogenetic Research on Classification of Indica and Japonica rice using SRAP, TRAP, SSR, ISSR markers Dai Xiaojun (College of Life Science HuNan Normal University, ChangSha, HuNan, 410081, China) 25 30 35 40 Abstract: Rice (Oryza sativa L.) is an important food crop, which is a constituent of more than 50% of the different varieties of food preparations in China. In order to exploit the utilization of heterosis between rice subspecies, 47 varieties of rice were classified into indica and japonica types firstly, using sequence-related amplified polymorphism (SRAP) and target region amplification polymorphism (TRAP) markers and then simple sequence repeat (SSR) and inter simple sequence repeat (ISSR) markers. Based on differences in the level of polymorphisms detected by the 4 markers—SRAP ISSR SSR TRAP. The average genetic similarity of SRAP is 0.70, TRAP is 0.74, SSR 0.75, ISSR 0.85. which showed highly significant correlation between different markers.The subspecies could be differentiated by cluster analysis while some plants were different in 4 Phylogenetic trees. The result indicated that SRAP and TRAP have potential application in rice research and SRAP has more differentiated ability between varieties that had a near relationship.The javanica rice Lemont and P002 were belong to indica clade. Some wild rice varieties characterized as indica-like or japonica-like in cluster analysis with different ma