大熊猫西氏贝蛔虫58.5KD重组抗原对猪蛔虫的交叉免疫保护研究.docVIP

 大熊猫西氏贝蛔虫 58.5KD 重组抗原对猪蛔 虫的交叉免疫保护研究# 5  摘要：目的 探究大熊猫西氏贝蛔虫（Baylisascaris schroedari）58.5KD 重组抗原免疫小鼠 对猪蛔虫（Ascaris suum）攻击感染的交叉保护效果。方法 1）用分子量为 58.5KD 的 Baylisascaris schroedari 重组抗原制剂经皮下分三次免疫 10 只小鼠，另设弗氏完全佐剂（10 只小鼠）和生理盐水（10 只小鼠）组作为对照。2）在第三次免疫后，所有实验小鼠用 L2 10 15 20 25 30 35 40 期 Ascaris suum 虫卵灌胃感染（4500 个/小鼠）。3）感染后第 7d 剖杀 18 只小鼠（每组随机 抽出 6 只），取出肝脏和肺脏采用贝尔曼法收集幼虫，在显微镜下观察并计数。4）用 ELISA 方法对免疫前后不同时间采集的小鼠血清进行 IgG、IgG1、IgG2、IgE、IgM 抗体检测，以 了解其抗体水平的动态变化，并比较各组实验动物血清抗体水平差异。结果 1)免疫组与生 理盐水对照组比较, 小鼠肝脏、肺脏内的幼虫数显著减少，其减虫率为 75.25%；弗氏完全 佐剂也具有一定的免疫效果，较西氏贝蛔虫 58.5KD 重组抗原制剂免疫效果差。2）免疫组 小鼠血清中 IgG、IgG1、IgG2 水平随初次免疫和加强免疫而持续上升，其水平高于弗氏完 全佐剂组与生理盐水组，统计学分析差异性显著（P0.05）。免疫组小鼠血清中 IgE、IgM 抗体水平未随重组蛋白注射出现明显增高，且免疫组、弗氏完全佐剂组、生理盐水组之间差 异性不显著（P0.05）。结论 Baylisascaris schroedari58.5KD 重组抗原能诱导小鼠产生对 Ascaris suum 攻击感染较强的保护作用。 关键词：西氏贝蛔虫；西氏贝蛔虫 58.5KD 重组抗原；交叉保护；猪蛔虫 中图分类号：S855.9 Cross-Protection against Ascaris suum Infection in Mice Immunized with Baylisascaris schroedari 58.5KD Recombinant Antigen SHEN Li, XIE Yue, YANG Guangyou (College of Veterinary Medicine, Sichuan Agricultural University, SiChuan Ya’an 625014) Abstract: Objective To investigate the cross-protection effect against Ascaris suum infection in mice immunized with B.schroedari recombinant 58.5KD antigen vaccine.Methods 1) 30 KM mice were divited into three groups,immunized with B.schroedari recombinant antigen vaccine, Freund’s complete adjuvant and physiological saline through hypodermic injection, respectively.2) After the 3rd immunization, every vaccinated mice underwent gavage of 4500 L2 A.suum infective eggs.3) 7days later after infection, 6 randomly chosen mice of every group were dissected to collect larvae from liver and lung through Baermannisation. The larvae were counted under microscope.4) IgG、IgG1、IgG2ɑ、IgE、IgM antibody tests were made on the mice serum collected at different time before and after immunization through ELISA. The difference of antibody level in the serum from different groups were compared as well. Results 1) Compared wit