壳聚糖_海藻酸钠微胶囊固定化木聚糖酶的研究.pdfVIP

, Jou n al of Anhu iAg .i Sci. 2009, 37( 16) : 7318 - 7319, 7325 李菲菲 夏蓉 - * 王 娜, 朱启忠 , 任延刚, 张瑞景, 高 原 (, 264209) [目的] 研究游离酶和壳聚糖- 海藻酸钠微胶囊 定化酶的酶学性质[方法 ]以壳聚糖和海藻酸钠为载体, 利用微胶囊技术 定木聚糖酶[结果]结果表明, 壳聚糖- 海藻酸钠微胶囊法 定化木聚糖酶的 定率为 39. 4% , 定化酶和游离酶的Km 值分别为 0. 765和 0. 687 g /L, 定化酶的热稳定性重复操作稳定性和贮藏稳定性有了明显提高[结论]采用壳聚糖- 海藻酸钠微胶囊法 定 化木聚糖酶具有一定的应用价值 海藻酸钠 ; 壳聚糖; 定化; 木聚糖酶 Q814 A 0517- 6611( 2009) 16- 07318- 02 EnzymaticCharactersofFreeXylanaseand ImmobilizedXylanase,Researchon theImmobilizedXylanasewithChitosan-sodium A-l ginateM icrocapsule ANGNa eta l (W e hi a iM a ine College of Shandong Un ive sity, W eihai, Shandong 264209) Abstract [ Objective] The enzymatic p ope ty of f ee Xy lanase and mi mob ilized xy lanase w as esea ched. [M ethod] The sodium alginate and chitosan being taken as ca ie, the m ic ocapsule techn ique w as applied to mi mobilize xy lanase. [ R esults] The esu lts ind icated that the ecove y pe centage of enzyme activ ity w as 39. 4% w hen the xy lanase w as mi mobilizedw ith the sodium alg inate-chitosanm ic ocapsule, and the Km value of mi m obilized xy lanase and f ee xy lanase w e e 0. 765 and 0. 687 g /L, espectively. And a lso, tempe atu e stab ility, ope ation sta- b ility and sto age stability o f mi mob ilized xy lanase w e e obviously mi p oved. [ Conclusion] Itw as concluded that the method of the sodium alg- inate-chitosan m ic ocapsule mi mobilizing xy lanase had the va lue in indust y. Keywords Sodium alginate; Ch itosan; mI mobilization; Xy lanase , 15% ~ 10 h0. 1 m ol/L NaC l 0. 1 [ 1] 30% , m ol/L pH 5. 5N aA cpH 5. 0HAc , , 4 e [2] , 1. 3 1 m l, , (A ; ,