酸枣仁总皂苷和总黄酮提取工艺优化.docxVIP

PAGEI

酸枣仁总皂苷和总黄酮提取工艺优化

【摘要】目的：以酸枣仁作为研究对象，通过Box-Behnken响应面法对酸枣仁总皂苷和总黄酮的提取工艺同时进行优化。方法：使用紫外分光光度法分别测定酸枣仁中总皂苷和总黄酮的含量，在单因素试验中，对不同条件下酸枣仁总皂苷和总黄酮提取率的影响趋势进行初步研究，采用Box-Behnken响应面分析方法考察4个对提取率影响较大的因素，并同时优化酸枣仁总皂苷和总黄酮的提取条件。结果：酸枣仁总皂苷和总黄酮的最优提取条件为：预浸30min、乙醇体积分数为80%、液料比为12.5:1mL/g、超声温度为59℃、超声功率为200W、超声时间为45min、提取1次，经实验得出酸枣仁总皂苷的提取率为0.993%，酸枣仁总黄酮的提取率为0.099%。结论：本实验中Box-Behnken响应面分析方法得到的酸枣仁总皂苷和总黄酮最佳提取工艺高效，稳定性良好，准确可靠。

【关键词】酸枣仁；总皂苷；总黄酮；超声波提取；响应面法

PAGEII

OptimizationofextractiontechnologyoftotalsaponinsandtotalflavonoidsfromSemenZiziphiSpinosae

[Abstract]Objective:ThepurposeofthisstudywastoenhancetheextractionprocessofSemenZiziphiSpinosaethroughtheutilizationoftheBox-Behnkenresponsesurfacemethod.Methods:ThestudyaimedtodeterminethetotalsaponinsandtotalflavonoidsinSemenZiziphiSpinosaebyultravioletspectrophotometry.ThesinglefactorexperimentwasconductedtoinvestigatetheextractionrateofthesecompoundsunderdifferentconditionsinSemenZiziphiSpinosae.ThestudyutilizedtheBox-Behnkenresponsesurfaceanalysismethodtoexaminefourfactorsthatsignificantlyaffectedtheextractionrates.ThegoalwastooptimizetheextractionconditionsfortotalsaponinsandtotalflavonoidsfromSemenZiziphiSpinosae.Results:TheidealconditionsforextractingtotalsaponinsandtotalflavonoidsfromSemenZiziphiSpinosaewereasfollows:thepresoaktimewas30min,theconcentrationofalcoholprecipitationwas80%,theliquid-to-materialratiowas12.5:1mL/g,theultrasonictemperaturewas59°C,theultrasonicpowerwas200W,theultrasonictimewas45min.Extractionshouldbeperformedoncetoachieveoptimalresults.Thestudyfoundthattheextractionrateoftotalsaponinswas0.993%andtheextractionrateoftotalflavonoidswas0.099%.Conclusion:Inthisexperiment,theBox-BehnkenresponsesurfaceanalysismethodyieldsanoptimumextractionprocessfortotalsaponinsandtotalflavonoidsfromSemenZiziph