微流体芯片资料.pptxVIP

微流体芯片资料第1页/共31页 ContentsBackground ExperimentABSTRACTDiscussion第2页/共31页 ABSTRACTMicrodroplets o?er unique compartments for accommodating a large number of chemical and biological reactions in tiny volume with precise control. A major concern in droplet-based micro?uidics is the di?culty to address droplets individually and achieve high throughput at the same time.第3页/共31页 Here, we have combined an improved cartridge sampling technique with a micro?uidic chip to perform droplet screenings and aggressive reaction with reagent consumption. Our combined device provides a simple model to utilize multiple droplets for various reactions with low reagent consumption and high throughput.第4页/共31页 第5页/共31页 BackgroundA large number of reactions can be independently miniaturized into tiny droplets ranging from nanoliter to picoliter sizes without having to increase the chip size and complexity. Microfluidic chips have provided a powerful platform for accomplishing droplet formation and manipulation within one chip of only a few square centimeters in size. AdvantagesOver the past few years, droplet-based micro?uidics has attracted increasing attention.第6页/共31页 Background Droplet generation for multiple samples is limited by the “world-to-chip” interface problem and cannot be addressed individually Droplet generation and manipulation functions within one chip can easily interfere with each other and are difficult To balance, despite being separated by a long, winding channel. Drawback Passive droplet formation methods:第7页/共31页 InnovateThis context shows a hybrid device :the cartridge droplet generation technique combined with a microfluidic chip. They improved the cartridge technique with a novel movement scheme: the capillary, instead of the multiwell plate,was moved by a digital and automatic manipulator to aspirate different samples or the covering oil.第8页/共31页 The hybrid droplet device for generation and manipulation consists of four major components . (a) manipulator(b)