浓缩丹参骨宝颗粒中水溶性有效成分的质量控制研究（职称论文资料）.docVIP

浓缩丹参骨宝颗粒中水溶性有效成分的质量控制研究（职称论文资料） 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：浓缩丹参骨宝颗粒中水溶性有效成分的质量控制研究 1 1 仪器和试药 3 2 方法与结果 3 1.供试品溶液；2.丹酚酸B对照品溶液；3.阴性对照溶液 4 3 讨论 6 文2：超声法提取促生长散中水溶性成分的分析 7 1 实验材料 8 2 实验方法 8 3 结果 10 4 结论 11 参考文摘引言： 11 原创性声明（模板） 12 文章致谢（模板） 13 正文 浓缩丹参骨宝颗粒中水溶性有效成分的质量控制研究（职称论文资料） 文1：浓缩丹参骨宝颗粒中水溶性有效成分的质量控制研究 Quality control for hydrophilic active components in Dahen Gubao Granules JIANG Guixiang1， LU Yingnian2， FAN Shijin2， ZHONG Liping2， CUI Liao2 Shuanglin Biopharmacy Co. Ltd， the Engineering Center of Zhanjiang Biopharmacy， Guangdong Province， Zhanjang 524005， China; Medical College， Guangdong Key Lab for Research and Development of Natural Drugs， Guangdong Province， Zhanjang 524023， China [Abstract] Objective: To establish a quality control method for hydrophilic active components in Dahen Gubao Granules. Methods: Salvia miltiorrhiza Bge. was identified by thin-layer chromatography， and the deteminatio of Daheu and Salvianic acid B were carried out by high performance liquid chromatography. Separatio were performed on a Hypeil ODS C18 ( mm×250 mm， 5 μm) column， the mobile phase was the mixed solvents of methnol-water-glacial acetic acid (55∶45∶， V/V) which set at a flow rate of ml/min， and the wave length for UV detection was 281 nm. The temperature was maintained at 35℃. Results: The TLC method was suitable for the identification of Salvia miltiorrhiza Bge.. The linearities for Daheu and Salvianic acid B were obtained with in the range of μg/ml and μg/ml (r= 8 and r= 9)， and the average recoveries were % and % respectively. Conclusion: This method is reliable and accurate that it can be used as a quality control method of hydrophilic active components in Dahen Gubao Granules. [Key words] Daheu; Salvianic acid B; Quality Control 丹参为唇形科鼠尾草植物的干燥根部，丹参的有效成分包含脂溶性的蒽醌类成分和水溶性的酚酸类成分[1]。丹参中的有效成分能增加冠脉血流量，降血脂，抑制血栓形成，改善微循环[2-3]，临床常用于心血管疾病的治疗，如市售的复方丹参片、丹参滴丸等。现代药理学研究发现，丹参水溶性活性成分具有抗骨质疏松的作用[4]，其中，丹参素可有效预防糖皮质激素引起的大鼠骨质疏松，丹参素可增加大鼠成