设计说明书 林蛙皮中多糖提取方法的比较.pdfVIP

中国林蛙 （Rana chensinensis David ）又名蛤什蟆，属两栖纲、无尾目、蛙科、 蛙属，是集药用、食补、 容功能于一体的珍稀两栖类动物，具有很高的应用价值 和开发潜力。 本文以东北林蛙皮为实验材料，以多糖的提取率为考察指标，将林蛙皮粉碎， 过筛，活性炭脱腥，比较热水提取法、碱提取法、酶提取法三种不同提取方法的提 取效果，并用苯酚-硫酸法测定多糖含量。实验结果表明，酶法提取效果最佳，其次 是热水提取法和碱提取法。采用正交试验法对酶法提取多糖的工艺进行优化，得出 其最佳提取工艺条件为：酶用量2% ，胃蛋白酶与胰蛋白酶比2:1，提取温度50℃， 提取时间7h ，多糖提取率为0.91% 。 ：林蛙皮；多糖；酶法；正交试验 1 Abstract Chinese forest frog ( Rana chensinensis David ) a a Hashima, belonging to the Amphibia,Anura, frog, Rana, is a set of medical, food, cosmetic functions in one of the rare amphibian animal, has very high value for application and promising future. The s in of Rana dybows ii is used as experimental material, with the extraction rate of polysaccharide as an index. Crushing the forest frog s in , sieving it, activated carbon deodorization, compared with hot water extraction, al ali extraction, enzyme extraction of three different extraction methods, and by phenol sulfuric acid method for the determination of polysaccharide content. The experimental shows that enzymatic extraction has the best effect, , followed by hot water extraction and al ali extraction. Using the orthogonal test method on enzyme extraction polysaccharide technology optimization, the optimum extraction conditions for enzyme dosage:2% enzyme, pepsin and trypsin than 2:1, extraction temperature is 50 extraction time is 7h, polysaccharides extraction rate is 0.91%. Key words: forest frog s in; polysaccharide; enzyme method; orthogonal test 1