小麦细胞核仁中DNA原位位置与rRNA基因转录位点的研究-遗传.pdf

HEREDITAS (Beijing) 2008 2 , 30(2): 231―236 ISSN 0253-9772 研究报告 DOI: 10.3724/SP.J.1005.2008.00231 DNA rRNA 1 2 3 赫杰 , 陶伟, 郝水 1. , 150001; 2. , 100871; 3. , 130024 : 以小麦细胞为研究材料, 应用常规电子显微镜技术和DNA 细胞化学特异染色NAMA-Ur 技术, 在原位水 平对核仁中 DNA 的分布和特征进行了直观的观察。结果表明, 小麦细胞核仁中 DNA 位于纤维中心(Fibrillar Centers, FC) 、致密纤维组分(Dense Fibrillar Component, DFC) 以及两者的过渡区域, 并呈现出环绕FC 排布的构 型; 应用RNP 优先染色(Benhard staining)技术分析了核仁中RNP 的分布及其原位位置, 直观的显示了小麦细胞 核仁中RNP 颗粒主要集中在 FC 与DFC 的过渡区域及DFC 和颗粒组分(Granular Component, GC) 中; 并且在 FC 与DFC 的过渡区域, 它们不太均匀也不太连续地半围绕着FC 而排布; 进一步借助于RNA/DNA 杂合体抗体 在原位水平标记和分析了细胞核仁中活跃基因转录的精细位点, 结果表明小麦细胞核仁rRNA 基因的转录位点 位于FC 与DFC 的过渡区域及DFC 中。 : DNA 特异染色; RNP 优先染色; RNA /DNA 杂合体; 小麦 Localization of nucleolar DNA and transcription sites of rRNA genes in situ in wheat cells 1 2 3 HE Jie , TAO Wei , HAO Shui 1. Department of Life Science and Engineering , Harbin Institute of Technology, Harbin 150001, China; 2. College of Life Sciences, Peking University, Beijing 100871, China; 3. Institute of Genetics and Cytology , Northeast Normal University , Changchun 130024, China Abstract: By using the conventional electron microscopic technique and DNA specific cytochemical staining method (NAMA-Ur), we directly observed the arrangement and location of intranucleolar DNA in situ in wheat (Triticum aestivum L.) cells. The results showed that nucleolar DNA was found in Fibrillar Centers (FC), Dense Fibrillar Component (DFC) and the transitional region between FC and DFC. Moreover, the nucleolar DNA was distributed along th