数字聚合酶链式反应技术在食品安全检测领域的研究应用进展-食品科学.pdf

※专题论述 食品科学 2016, Vol.37, No.17 275 数字聚合酶链式反应技术在食品安全检测 领域的研究应用进展 1 2 1 1 3 1,* 刘 津 ，刘二龙，谢 力，李志勇，相大鹏，高东微 （1.广东检验检疫技术中心，广东省动植物与食品进出口技术措施研究重点实验室，广东 广州 510623 ； 2.黄埔出入境检验检疫局，广东 广州 510700 ；3.广东出入境检验检疫局，广东 广州 510623 ） 摘  要：数字聚合酶链式反应（digital polymerase chain reaction，dPCR ）作为一项新兴的基于单分子目标基因扩增 的绝对定量技术，促进了现代分子生物学在精准定量检测方面的发展和应用。本文综述了商业化dPCR技术在转基 因成分、动物源性成分、食源性致病微生物定量检测等食品安全领域的研究进展，讨论了dPCR应用中技术难题的 解决，并在此基础上展望了该技术在食品安全检测领域的发展前景。 关键词：数字聚合酶链式反应；食品安全；绝对定量；检测 Progress in Research and Application of Digital Polymerase Chain Reaction (dPCR) in Food Safety Detection 1 2 1 1 3 1,* LIU Jin , LIU Erlong , XIE Li , LI Zhiyong , XIANG Dapeng , GAO Dongwei (1. Guangdong Inspection and Quarantine Technology Center, Guangdong Key Laboratory of Import and Export Technical Measures of Animal, Plant and Food, Guangzhou 510623, China; 2. Huangpu Entry-Exit Inspection and Quarantine Bureau, Guangzhou 510700, China; 3. Guangdong Entry-Exit Inspection and Quarantine Bureau, Guangzhou 510623, China) Abstract: Digital polymerase chain reaction (dPCR), which emerges as a new absolute DNA quantification technique based on monomolecular target gene amplification, can promote the development and application of modern molecular biology in accurate quantitative detection. A review of the literature on commercial dPCR applied in food safety detection is given in this paper, including quantitative detection of genetically modified organisms, animal derived ingredients and foodborne pathogenic microorganisms. A discussion on the solution of technical problems using dPCR is provided as well.