光谱仪控制、采集部分的改进设计及荧光光谱技术用于肝病诊断的研究-光学工程专业论文.docxVIP

摘要摘要 摘要 摘要 论文应用荧光光谱技术，对肝纤维化小鼠及肝癌患者的血清进行了光谱分 析，试图通过血清的自体荧光光谱找到一些有价值的数据，以用于肝病的诊断 特别是早期诊断。 文章综述了荧光光谱技术在医学诊断上的应用及其发展现状，对荧光光谱 和拉曼光谱的基本原理进行了简明阐述，并探讨了一些实验因素对荧光光谱的 影响；鉴于原有实验设备存在的缺点，论文设计并编写了用于单色仪控制及数 据采集、处理的应用软件，实现了各种实验操作的计算机控制，并对改进后的 装置进行了测试。 论文用氩离子激光器作为激发光源，对肝纤维化小鼠及肝癌患者的血清荧 光光谱进行了研究。通过对48例实验用小鼠血清样品的检测，得到了其光谱 的基本特征，结果表明肝纤维化发展不同时期的血清荧光光谱之间存在差别， 但并不非常明显；对21例肝癌患者血清的研究发现，肝癌患者的血清荧光一 拉曼光谱与以往检测的其它癌症相比，有一些共同的特点，但同时也表现出很 大的差异，以前得到的判据用于肝癌的诊断效果不佳，这提示不同的癌症可能 需分别研究以寻找更为准确的诊断标准。 本论文对激光诱导荧光方法用于肝纤维化的诊断进行了尝试，并对肝癌患 者的血清荧光一拉曼光谱进行了研究，论文的实验结果对以后的工作有着重要 的参考价值。 关键词：荧光光谱、拉曼光谱、肝纤维化、肝癌、血清、诊断 II ABSTRACTABSTRACT ABSTRACT ABSTRACT Optical spectroscopy for diagnosis of liver diseases was investigated in this PaDeL Laser—induced fluorescence and Raman spectra were measured from blood seilin of liver cancer sufferers and rats in different stages of hepatic fibrosis．in an attempt to find some clues which carl be used in diagnosis． Using 488．0rim and 514．5nm excitation of an Ar．ion Iaser,we studied twenty．one sernm samples of liver cancer sufferers．Red shifl of fluorescence peak and decrease of fluorescence intensity were observed after samples were radiated by laser．Of all the samples，two cases are characterized by three sharp distinct Ranlan Deaks．Such results are consistent with what we obtained fr0Ill other cancer cases in the past However,there are also many differences between them．The diagnostic criteria drawn froln other cancer cases cannot distingnish 1iver cancer from normal one effectively．It indicates that different cancers should be investigated respectively in order to find more accurate diagnostic criteria． In the study of hepatic fibrosis，spectral data were obtained from forty—eight rat serklm samples．According to pathological result of liver tissue，samples were classified into six kinds．The spectral features of each kind were analysed．Result showed that there was some difference between them，but not distinct． In addition．an application software was designed and accomplished in this article