灵芝肽对小鼠半乳糖胺致肝损伤的保护作用.PDF

416 2008, Vol. 29, No. 05 食品科学 ※营养卫生 灵芝肽对小鼠半乳糖胺致肝损伤的保护作用 石燕玲，何 慧*，梁润生，张 胜，郭 辉，黄迪惠 (华中农业大学食品科技学院，湖北 武汉 430070) 摘 要：目的：研究灵芝肽(GLP)对D-半乳糖胺(D-Gal)诱导的小鼠化学性肝损伤的保护作用。方法：将小鼠随机 分成正常组、模型组、GLP 低、中、高剂量组，每天灌胃一次，GLP 组分别灌胃GLP 60、120、180mg/kg bw， 正常组、模型组灌胃等量生理盐水，2周后模型组和GLP组腹腔内注射D-Gal (750mg/kg bw)，诱导小鼠肝损伤。 通过试剂盒的方法分别检测各组小鼠血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)活力，肝组织中丙二醛(MDA)、 谷胱甘肽(GSH)含量及超氧化物歧化酶(SOD)活力；并进行肝组织病理学镜检。结果：与模型组比，以180mg/kg bw 给予GLP，可显著降低小鼠血清ALT、AST 的活性和肝组织中MDA 含量，提高肝组织中SOD 的活力与GSH 的 含量(p＜0.05～0.01)，肝组织病理学镜检结果显示已接近正常组。结论：GLP对D-Gal诱导的小鼠肝损伤有很好 的保护作用。 关键词：灵芝肽；D - 氨基半乳糖；肝损伤；保肝 Hepatoprotective Effects of Ganoderma lucidum Peptides Against D-galactosamine-induced Liver Injury in Mice SHI Yan-ling，HE Hui*，LIANG Run-sheng，ZHANG Sheng，GUO Hui，HUANG Di-hui (College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China) Abstract ：Objective: To research the hepatoprotective effects of Ganoderma lucidum peptides (GLP) against D-galactosamine (D-Gal)induced liver damage in mice. Method ：Mice were randomly divided into five groups, namely normal, model and three GLP-treated groups. GLP-treated groups were daily given (ig) the doses at 60, 120 and 180 mg GLP /kg bw respectively. Normal and model groups simultaneously received the same amount of physiological saline for 2 weeks. Then the mice from model group and GLP-treated groups were treated with 750 mg/kg bw D-Gal (ip). Aspartate transaminase/alanine transaminase (AST/ALT) activities in serum, malondialdehyde/glutathione (MDA/GSH) levels and superoxidedismutase (SOD) activity in liver were measured by the detection kits according to the manufacturer’s protocols. The liver histopathological characters were obs