苯酚对斑马鱼行为学影响及毒理学效应研究市政工程专业论文.docxVIP

山东建筑大学硕士学位论文 山东建筑大学硕士学位论文 (4)进行了苯酚对斑马鱼毒理学效应的研究。通过考察试验组斑马鱼体内ATP酶、 乙酰胆碱酯酶(ACllE)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性的变化，探 究污染物胁迫下斑马鱼运动行为学变化与毒理学效应的相关性。由试验可知，苯酚胁迫 初期(6h内)，与对照组相比，低浓度(o．18、o．9mg／L)组ATP酶、AChE、SOD、CAT 活性分别增加了10．18％、20％、O．5．8％，呈现“毒性兴奋效应。 短时间胁迫下，苯酚 可诱导酶活性，随染毒时间延长，诱导作用减弱，甚至表现出抑制作用；随苯酚浓度增 加，ATP酶、AChE活力逐渐降低，SOD、CAT活力逐渐上升。由于反应的滞后作用， 0．18．36mg／L六个试验组CAT活性达到峰值的时间比SOD滞后约24h。各种生物酶活性 受苯酚影响，导致神经系统、大脑、肌肉等器官功发生改变，进而影响动物的行为变化， 斑马鱼的运动行为强度与体内酶活性之间存在明显剂量效应关系。 关键词：水质预警，斑马鱼，苯酚，运动行为学，毒理学 II 万方数据 山东建筑大学硕士学位论文Experimental 山东建筑大学硕士学位论文 Experimental Study on Behavior and Toxicological Effects of Phenol on Zebrafish Du Ju删Uarl(Municipal Engineering) Directed by Professor Li Mei ABSTRACT With the accelerated process of industrialization,the types and quantities of organic matter is increasing,and not only do organic pollutions cause water environmental degradation，but also risk human and aquatic organisms health．Zebrafish as a model organism，phenol contaminated water，with water quality and safety line biological early warning system(BEWs)and water toxicity bio-line monitor(RTB)，zebrafish locomotor activity changes are monitored real-time online under the stress of phenol，and combined with toxicological effects of zebrafish to analyze the correlation between the zebrafish behavior changes and toxicology index after exposuring to phen01．Experimental study contents and conclusions are as follows： (1)Acute toxicity experimental studys of phenol on zebrafish were carryed out with semi-static test method，obtaining LCso of different times．The results showed that phenol was a medium toxic substance to zebrafish，LCso．sh，LCso．96hwere 18．548，13．554mg／L，and safe concentration was 1．355mg／Lof phenol to zebrafish；compared with the LC50-96h of phenol to other fishes，the tolerance abilities of phenol to zebrafish were lower,and the sensitivities were higher．Under 30，40，50mg／L phenol stress，the time of the zebrafish mortality rate reached 1 00％were 48，1 2，1 2h，and 1 2，24，48，96h mortality rate waere 53％，6