低氧检测用双异核金属配合物探针研究应用化学专业论文.docxVIP

低氧检测用双异核金属配合物探针研究Study 低氧检测用双异核金属配合物探针研究 Study on Heterodinuclear Metal Complex Probe for Detecting Hypoxia Abstract Molecular Oxygen(dioxygen)is one ofthe pivotal metabolites in aerobic organisms，and is imperative for maintaining the vital activity in almost all living cells．Oxygen deprivation (hypoxia)，which is the result of inadequate oxygen supply,is closely connected with various diseases and occurs in tumor microenvironments．Hypoxia is a distinguished feature of malignant solid tumors，and the exploration of sensors which can accurately diagnose the oxygen level will be beneficial to estimate the malignant degree of tumor and subsequently execute more effective personalized treatment．Phosphorescence imaging of metal coordination complexes have attracted a great deal of attention because of their various advantages，which include high Stokes shifts，color-tunable luminescence，pronounced photostability，long emission lifetimes．Since the ground state of 02 is a triplet state，the emission of triplet excked state of the transition metal complexes can he quenched by collision with 02，enabling the metal complexes to monitor the oxygen content．However， majority of metal complex luminescence probes monitor the oxygen content with the intensity variation at one wavelength,which can he affected by the concentration of probes，exciting light intensity，and instrument performance．To overcome these problems，two metal complexes were synthesized with dual emission，and ratiometric monitored the oxygen variation in living systems． The heterodinuclear ruthenium and iridium metal complex 【Ru(bpy)3一(CH2)lO·Ir(F2ppy)2(bpy)]”(RuIr)Was employed to ratiometric monitor oxygen fluctuation in cells and mouse．There Was efficient F6rster resonance energy transfer(LRET) in[Ru(bpy)3一(CH2)10-Ir(F2ppy)2(bpy)]计，and[Ir(F2ppy)2(bpy)]+transfered majority of energy to[Ru(bpy)3]”，making the luminescence of[Ir(F2ppy)2(bpy)]+weak and insensitive to oxygen,while the luminescence of[Ru(bpy)3]计was stong and s