泰乐菌素降解菌株的生理生化特性研究.doc

PAGE 泰乐菌素降解菌株的生理生化特性研究 ****大学化学化学化工学院（制药工程）专业 **** 摘要：为了研究泰乐菌素降解菌株的生理生化特性，实验中采用YPD培养基在厌氧条件下对降解菌进行培养，并观察菌落生长形态、颜色及大小；再取部分降解菌进行革兰氏染色及瑞氏染色，并观察染色情况。结果表明：降解菌B5，Z1，S3均为球菌，且均为厌氧菌。经染色后均呈紫色为革兰氏阳性菌。瑞氏染色表明S3为嗜酸性菌，降解菌Z1，B5为嗜碱性菌。 关键词：降解菌株 特性 革兰氏染色 瑞氏染色 Study on the characteristics of degrading bacteria which act on tylosin in physiology and biochemistry Abstract:In order to learn about the characteristics of degrading bacteria which act on tylosin in physiology and biochemistry ，we adapted YPD substrate to cultivate the degrading bacteria,then observed the state of its growth,color and size,some part of the degrading bacteria were given Grams staining and Wrights staining to show the conclusion.The result shows that:the degrading bacteria B5,Z1,S3 are all coccus,anaerobic. After the dyeing,the bacteria turned into purple shows it is Gram-positive bacteria.Wrights staining Show that the S3 is diaacidophilu,,Z1,B5 are alkaliphlies. Key words: degrading bacteria ,characteristics, Grams staining ,Wrights staining 目 录 TOC \o 1-3 \h \u HYPERLINK \l _Toc229457656 第一章 引言 PAGEREF _Toc229457656 \h 1 HYPERLINK \l _Toc229457657 1.1研究综述 PAGEREF _Toc229457657 \h 1 HYPERLINK \l _Toc229457658 1.2研究目的及意义 PAGEREF _Toc229457658 \h 3 HYPERLINK \l _Toc229457659 第二章 试验内容 PAGEREF _Toc229457659 \h 4 HYPERLINK \l _Toc229457660 2.1细菌革兰氏染色 PAGEREF _Toc229457660 \h 4 HYPERLINK \l _Toc229457661 2.1.1供试菌种 PAGEREF _Toc229457661 \h 4 HYPERLINK \l _Toc229457662 2.1.2染色液和试剂 PAGEREF _Toc229457662 \h 4 HYPERLINK \l _Toc229457663 2.1.3器材 PAGEREF _Toc229457663 \h 4 HYPERLINK \l _Toc229457664 2.1.4 试验步骤 PAGEREF _Toc229457664 \h 4 HYPERLINK \l _Toc229457665 2.2对氧的需求鉴定及菌落形态 PAGEREF _Toc229457665 \h 7 HYPERLINK \l _Toc229457666 2.2.1供试菌种 PAGEREF _Toc229457666 \h 7 HYPERLINK \l _Toc229457667 2.2.2 YPD培养基 PAGEREF _Toc229457667 \h 7 HYPERLINK \l _Toc229457668 2.2.3器材 PAGEREF _Toc229457668 \h 7 HYPERLINK \l _Toc229457669 2.2.4实验步骤 PAGEREF _Toc229457669 \h 7 HYPERLINK \l _Toc