分子生物学杨洋翻译.pptx

2018/11/25;2018/11/25;2018/11/25;CHAPTER 7 Translation （第七章 翻译）;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;Finding of Ribosome binding site (RBS) or SD-sequence;Model: R17 phage infects E. coli;;If the 30S subunit is from E.coli, phage coat protein gene can be translated;?;30S subunit was further dissociated into RNA and protein ;;More powerful experimental evidence for the importance of SD-sequence ;;;Phenomenon （现象）;Question: how can the cell recognize the correct start codon ? ;2018/11/25;2018/11/25;2018/11/25;2018/11/25;How was the Kozak sequence found? ;;Kozak sequence：5-G/ANNAUGG-3;Experimental evidence for proving the importance of the Kozak sequence: ;2018/11/25;Idea of mutation (genetic method);Design of the experiment;5-G/ANNATGG; G/ANNATGG;2018/11/25;Poly-A in the 3’ end promotes the efficient recycling of ribosomes, thus increasing the translation efficiency ;2018/11/25;2018/11/25;2018/11/25;The story of finding tRNA;;2018/11/25;The research work of Zamecnik in 1957;This RNA could be coupled to amino acid;; 牛牛文库文档分享;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;Why the aminoacyl tRNA synthetase must recognize the amino acid and tRNA with very high accuracy and fidelity?;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25; 牛牛文库文档分享; 牛牛文库文档分享;;; 牛牛文库文档分享; 牛牛文库文档分享;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25;核糖体RNA负责催化肽基转移酶反应;2018/11/25;2018/11/25;2018/11/25;2018/11/25;2018/11/25