地黄试管块根诱导条件优化及其RNA的抽提植物学专业论文.docxVIP

PAGE PAGE IV CTAB and the RNAiso plus, were used to extract the total RNA from R. glutinosa. Then the results of the extraction were compared and analyzed by the production, electrophoresis detection and RT-PCR verification. Results showed that the total RNA extracted by bentonite method were clear and no dispersion, the integrity of the RNA was well, and there was no obvious contamination with DNA andother impuritie, which was applied to RT-PCR test. Key Words：Rehmannia glutinosa; Stem; Leaf; In vitro tuberous root; Catalpol; RNA 缩略词表 简写符号 英文全称 中文全称 ATP Adenosine Triphosphate 腺嘌呤核苷三磷酸 6-BA 6-Benzyladenine 6-苄基腺嘌呤 CTAB Hexadecyl Trimethyl Ammonium Bromide 十六烷基三甲基溴化铵 2,4-D 2,4-DichloroPhenoxyacetic acid 2,4-二氯苯氧乙酸 DEPC DiEthyPyroCarbonate 焦碳酸二乙酯 DNA Deoxyribonucleic Acid 脱氧核糖核酸 dNTP Deoxy-ribonucleoside Triphosphate 脱氧核糖三磷酸 EDTA Ethylenediaminetetraacetic acid 乙二胺四乙酸 ESTs Espressed sequence tag sequences 表达序列标签序列 GA Gibberellic acid 赤霉素 IAA Indole-3-acetic Acid 吲哚-3-乙酸 IBA Indole-3-butytric Acid 吲哚丁酸 KT Kinetin 激动素 MeJA mRNA Jasmonic Acid Methyl Ester Messenger ribonoucleic acid 茉莉酸甲酯 信使核糖核酸 MS Murashige and Skoog(1962) MS 基本培养基 NAA PP333 Naphthaleneacetic Acid Paclobutrazol 萘乙酸 多效唑 PVP RNA RNase Poly Vinyl Pyrrolidone Ribonucleic Acid Ribonuclease 聚乙烯基吡咯烷酮 核糖核酸 核糖核酸酶 RT-PCR Tris Reverse Transcription PCR Trihydroxymethyl Aminomethane 反转录 RCR 三羟甲基氨基甲烷 目录 HYPERLINK \l _bookmark0 第一章 绪论1 HYPERLINK \l _bookmark1 1.1 地黄研究概况1 HYPERLINK \l _bookmark2 1.1.1 地黄化学成分的研究1 HYPERLINK \l _bookmark3 1.1.2 地黄药理作用研究进展2 HYPERLINK \l _bookmark4 1.1.3 地黄组织培养的研究3 HYPERLINK \l _bookmark5 1.2 植物地下贮藏器官离体诱导的研究进展5 HYPERLINK \l _bookmark6 1.2.1 植物生长物质与器官离体诱导5 HYPERLINK \l _bookmark7 1.2.2 碳源和无机元素与器官离体诱导6 HYPERLINK \l _bookmark8 1.3 地黄分子生物学的研究6 HYPERLINK \l _bookmark9 1.4 植物 RNA 抽提方法的研究 7 HYPERLINK \l _bookmark10 1.5 研究目的和意义7 HYPERLINK \l _bookmark11 1.6 技术路线9 HYPERLINK \l _bookmark12 第二章 茎段诱导地黄试管块根技术优化的研究10 HYPERLINK \l _bookmark13 2.1 材料与方法10 HYPERLINK \l _bookmark14 2.