钙结合蛋白S100A9在小鼠铜绿假单胞菌肺炎中的表达及意义-内科学专业论文.docxVIP

PAGE PAGE 5 Calcium binding protein expression and significance of S100A9 in lung tissue in mice infected by Pseudomonas aeruginosa Abstract Objective: the pathological changes of lung tissue in mice with Pseudomonas aeruginosa pneumonia infections observed and corresponding expression in lung tissue of calcium binding protein S100A9RNA, and determine S100A9 calcium binding protein content and common inflammatory factor TNF-a in mouse blood, status and role of calcium binding protein S100A9 in mice induced by Pseudomonas aeruginosa lung infection in the. Methods: this experiment requires 36 mice, were randomly divided into control group, model group of 12 3H 12, 6h 12 rats in model group, the three group. Through the ring capsular puncture injection of Pseudomonas aeruginosa bacteria to establish the model of pulmonary infection. 36 clean mice were randomly divided into blank control group (12 rats, referred to as the 1 group, ring capsule injection of saline, die molding process 2), model group (24 rats, by Pseudomonas aeruginosa bacteria liquid, ring capsule injection of equivalent 2 Maxwell unit concentrations were divided into 3H and 9h two time. The modeling of death 5), respectively, in 3 hours and 9 hours of 2 time points were 9 and 10 respectively. The mice right lower lobe lung tissues were observed with HE staining to observe pathological, blank control group, pathological changes in 3H model group and 9h model group; yield of S100A9mRNAPCR three in lung tissues of mice in the reaction process using ordinary PCR detection system for real-time monitoring, the final analysis of the experimental data, the difference and compared between the three groups mice produced S100A9mRNA. And synchronization using double antibody sandwich enzyme linked immunosorbent assay (ELISA) method were used to determine the three blood of mice in group S100A9 and TNF-a content, S100A9 calcium binding protein differences in mouse blood and related department an