复方苦蛇乳膏治疗慢性湿疹的临床和实验研究-中医外科学专业论文.docx

成都中医药大学 成都中医药大学 2006 级博士学位论文 - PAGE - PAGE 4 exudation of capillary vessel of skin on rate and itch in guinea pigs induced by histamine, inhibit the increase of capillary permeability caused by glacial acetic acid in mice ,significantly reduce the degree of tumefaction in mice with auricle by dimethylbenzene,but no obvious effect on tumefaction in feet by carrageenan. In the electronic microscope ,ultrastructure of skin of chronic eczema were observed and showed less damage after the treatment of KuShe compound cream. And it can reduce serum levels of IFN - г, IL - 18, IL – 31of chronic eczema model of mice , raise serum levels of IL – 4; and significantly reduced semi-quantitative total score of AQP3 in chronic eczema skin tissues , intensity of dyeing is weak to moderate, mainly expressed in the stratum basal , rarely expressed in the stratum spinosum . Conclusion: clinical trials point out: KuShe compound creams have good efficacy and safety in the treatment of chronic eczema, the research of animal experiments suggests that KuShe compound creams have excellent effects of anti-inflammatory and antiallergic, has the fine role of prevention and cure on chronic eczema,which provides a good experimental basis.In addition, we speculate that the mechanism of KuShe compound creams on chronic eczema is probably adjusted dynamic balance of T helper cells (Th1/Th2), restrain expression of AQP3 in the stratum spinosum, granular and corneum. Key words: KuShe compound cream chronic eczema clinical study experiment study 缩略词表 英文缩写 英文全称 中文全称 TH Th1 Th2 CK T helper T helper 1 T helper 2 Cytokine T 辅助细胞 T 辅助细胞 1 T 辅助细胞 2 细胞因子 IFN-γ IL-4 IL-18 IL-31 AQP3 DNFB interferon-γ Interleukin-4 Interleukin-18 Interleukin-31 Aquaporin-3 Dinitrofluobenzene γ干扰素 白细胞介素－4 白细胞介素－18 白细胞介素－31 水通道蛋白 3 二硝基氟苯 DNCB Dinitrochlorobenzene 二硝基氯苯 DTH DelayedTyPeofHyPersensitivity 迟发性变态反应 ELISA Enzyme-linked Immunosorbnent Assay 酶联免疫吸附试验 - PAGE - PAGE 12 序 言 近年来，湿疹的发病率呈上升趋势，这可能与环境气候变化，饮食结构改