活性红4离子缔合分光光度法测定壳聚糖-食品科学.PDFVIP

※分析检测 食品科学 2010, Vol. 31, No. 16 229 白 研，陈 纯，陈芷仪，苏政权 * (广东药学院公共卫生学院，广东 广州 510310) ：在pH4.5 的B-R 缓冲溶液和HAc-NaAc 缓冲溶液中，活性红4 与壳聚糖的离子缔合物在 574nm 波长处的吸 光度与壳聚糖含量在 0～4.0 μg/mL 质量浓度范围内呈良好线性关系。在 B-R 缓冲溶液和HAc-NaAc 缓冲溶液中本方 2 2 法的回归方程分别为A=0.3434c －0.0355，R =0.9996 和A=0.3229c －0.03，R =0.9992 。两种缓冲体系测得结果无显著 性差异，且不受壳聚糖分子量大小的影响。以本法测定甲壳素胶囊中壳聚糖的含量，结果与该制剂的壳聚糖标示 量相符。样品测定精密度为 1.43%(n=6) ，平均回收率为 101.1%。该方法具有较强的选择性，适用于较复杂样品 中壳聚糖含量的测定。 ：壳聚糖；活性红 4 ；分光光度法 Spectrophotometric Determination of Chitosan Based on Ion Association Reaction with Reactive Red 4 BAI Yan ，CHEN Chun ，CHEN Zhi-yi ，SU Zheng-quan* (School of Public Health, Guangdong Pharmaceutical University, Guangzhou 510310, China) Abstract ：A sensitive and rapid analytical method was developed for the determination of chitosan based on the fact that the absorbance of reactive red 4/chitosan complex at 574 nm has linear relationship with the added concentration of chitosan in both B-R buffer solution (pH 4.5) and HAc-NaAc buffer solution (pH 4.5). The linear equation of the method was A = 0.3434c － 2 2 0.0355 (the unit of c: μg/mL, hereinafter the same) (R = 0.9996) in B-R buffer solution and A = 0.3229c －0.03 (R = 0.9992) in HAc-NaAc buffer solution with a linear range from 0 to 4.0 μg/mL. The determination results obtained using the above two kinds of buffer solutions had no significant difference and were not affected by the molecular weight of chitosan. This method was utilized to determine the content of chitosan in a commercial brand of chitosan capsules, and the resul