HPLC测定当归5种化学成分不定度评定.docVIP

HPLC测定当归5种化学成分不确定度评定 　　摘要：目的 建立高效液相色谱法（HPLC）同时测定当归中阿魏酸、正丁基苯酞、正丁烯基苯酞、Z-藁本内酯及亚油酸的不确定度评定方法。方法 采用HPLC同时测定当归中5种化学成分，分析检测过程中的各种不确定因素，并对各影响因素进行考察。结果 用HPLC测定当归中阿魏酸、正丁基苯酞、正丁烯基苯酞、Z-藁本内酯和亚油酸的合成标准不确定度分别为3.23×10-2、4.59×10-2、1.19×10-2、2.15×10-2和8.72×10-2，即当归中5种化学成分在测定中的不确定度大小依次为亚油酸正丁基苯酞阿魏酸Z-藁本内酯正丁烯基苯酞。结论 不确定度评定可为当归有效成分的定量研究提供一定参考。 中国论文网 /1/viewhtm　　关键词：当归；指标性成分；高效液相色谱；不确定度评定 　　DOI：10.3969/j.issn.1005-5304.2015.12.019 　　中图分类号：R284.1 文献标识码：A 文章编号：1005-5304（2015）12-0075-04 　　Evaluation of Uncertainty Measurement on 5 Kinds of Chemical Substance in Angelicae Sinensis Radix by HPLC DING Jun-xia1， GU Zhi-rong1， SUN Yu-jing1，2，3， WANG Ya-li1，2，3， ZHANG Ya-ya1， WANG Yao-peng1 （1.Center of Scientific Experiment， Gansu University of Chinese Medicine， Lanzhou 730000， China；2.Institute of Angelica Sinensis， Gansu University of Chinese Medicine， Lanzhou 730000， China；3.Key Laboratory for Traditional Chinese （Tibetan） Medicine Chemistry and Quality Control of Gansu University， Lanzhou 730000， China） 　　Abstract：Objective To establish a method for evaluating uncertainty of determining ferulic acid， 3-butylphthalide， Z-butylidenephthalide， Z-ligustilide and linolic acid in Angelicae Sinensis Radix by HPLC. Methods HPLC was used for simultaneously determining the 5 kinds of chemical substance in Angelicae Sinensis Radix， and various uncertain factors and these factors in the process of measurement were analyzed and investigated. Results The synthetical standard uncertainty of ferulic acid， 3-butylphthalide， Z-butylidenephthalide， Z-ligustilide and linolic acid in Angelicae Sinensis Radix measured by HPLC were 3.23×10-2， 4.59×10-2， 1.19×10-2， 2.15×10-2 and 8.72×10-2， respectively. The uncertainty of 5 kinds of chemical substance in Angelicae Sinensis Radix was linolic acid3-butylphthalideferulic acidZ-ligustilideZ-butylidenephthalide. Conclusion The evaluation of uncertainty measurement can be used to offer certain references for quantitative research on active ingredients in Angelicae S