Cytocompatibility of chitosan―based thermosensitive hydrogel to human perioontal ligament cell.docVIP

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Cytocompatibility of chitosan―based thermosensitive hydrogel to human perioontal ligament cell.doc

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Cytocompatibility of chitosan―based thermosensitive hydrogel to human perioontal ligament cell

Cytocompatibility of chitosan―based thermosensitive hydrogel to human periodontal ligament cell 　　【Abstract】Objective： To investigate the effect of thermosensitive chitosan/β-glycerophosphate （CS/β-GP） hydrogel on proliferation of human periodontal ligament cells （HPDLCs）. Methods： CS/β-GP were prepared into a thermosensitive hydrogel and its three-dimensional structure was observed under electron microscope. HPDLCs harvested and cultured in vitro were co-cultured with the thermosensitive CS/β-GP hydrogel. Growth of the cells in the hydrogel was observed with HE staining， and the effect of the extract on proliferation of HPDLCs was examined by CCK-8 assay. Results： Observations of SEM and HE staining showed that the thermosensitive CS/β-GP hydrogel was large in pore size and appropriate for cell growth. Different levels of CS/α，β-GP extracts could promote proliferation of HPDLCs. Conclusion： Thermosensitive CS/β-GP hydrogel can promote proliferation of HPDLCs and be a good carrier for periodontal tissue engineering because of its thermosensitivity. 中国论文网 /6/viewhtm 　　【key words 】 CS/β-GP PDLCs CCK8 　　【CLC】R722.12 【Document code】B【Article number】1004-4949（2015）03-0516-02 　　High incidence of periodontal disease may result in destruction of periodontal supporting tissues. PDLC is of multiple differentiation potential， and plays a key role in periodontal tissue regeneration. Modern periodontal tissue engineering is expected to solve this problem， by which a certain amount of PDLCs are inoculated into an appropriate scaffold and form a cell-scaffold complex for co-culture. HPDLCs harvested and cultured in vitro are to be co-cultured with CS/β-GP hydrogel to investigate the biocompatibility of CS/β-GP with PDLCs. 　　Main materials and equipment FBS， α-MEM， CS ， β-GP， Cell Counting Kit-8 ， HE Staining Kit ， magnetic stirrer ， scanning electron microscope， CO2 Incubator ， Thermo Scientific Microplate Reader. 　　Methods 　　Preparation and property detection o