MIAPARlettersupplmentary materialsdocmiapar补充materialsdoc信.docVIP

Supplementary Materials Minimum Information about a Protein Affinity Reagent (MIAPAR) The information required for the description of affinity reagent/target pairs can be divided into: that which identifies the source of the report, that which refers to the characteristics of the molecular entities involved, and that which demonstrates both their individual properties and when in association in a binder/target pair (i.e. a report of experimental data which provides additional information as to the specificity of a reagent). Information about an affinity reagent/target pair can be represented as a set of two molecular entities (affinity reagent and target) and a relation (binding between them). As a consequence the information to be considered can be split into three categories (Table 3). Header The header holds the essential administrative information required to identify the source of the affinity reagent description. It may refer to the organization producing the binder, the authors of a paper introducing a new binder, etc. Molecular entities Each entity is documented with identification information, structural and biophysical properties as well as experimental data reporting generation and characterization processes. Details of each experimental stage could be described using the relevant Minimum Information guidelines. More precisely, for the reagent being described, its nature and method of production should be recorded, as this has implications for its affinity, as well as size, valency and stability. Sufficient detail should be given so that a user can identify any factors in the process which may impact on its application and resulting experimental data, but not such that the methodology is immediately reproducible by a third party. Similarly, it is equally important that the exact details of a target against which an affinity reagent is raised or designed be fully stated, such that the user can understand the implications of this for reagent cross