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CONFOCAL MICROSCOPY amp; IMAGE ANALYSIS激光共聚焦显微镜ap；图像分析.doc

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CONFOCALMICROSCOPY

LEICA DMIRE2 MICROSCOPE MANUAL Confocal Microscopy Unit, Life Sciences Building, Cardiff School of Biosciences, Cardiff University, Cardiff CF10 3US. Tel: 02920 876611/ 875139 This manual was prepared by Dr Tony Hayes, 2005 (hayesaj@cardiff.ac.uk) TABLE OF CONTENTS ANATOMY OF the Leica DMIRE2 inverted microscope 3 INITIALISATION PROCEDURE 4 LEFT SIDE OF MICROSCOPE (DETAILED VIEW) 5 DMIRE2 FRONT panel 6 1. LCD display of microscope status 6 2. LCD display control buttons 7 3. L.E.D. panel showing active fluorescence cube 7 4. Filter cube selection switch 7 5. Mercury lamp shutter 8 6. Port selection switch 8 7. Tube lens 8 Contrast methods 9 BRIGHTFIELD 9 FLUORESCENCE 9 MAGNIFICATION 11 CHANGING OBJECTIVE 11 FOCUSSING 12 Scanning Mode 13 ANATOMY OF THE LEICA DMIRE2 INVERTED MICROSCOPE 1. Binocular phototube 16. Filters 2. Eyepiece adapter tube 17. Aperture diaphragm adjustment 3. Eyepieces 18. Lamp housing mount 4. Tube mount 19. Lamp housing 5. Tube port for photo/TV connect 20. Stage plate 6. Beam splitter 21. Analyser 7. Mains switch 22. Tube lens module 8. Brightness adjustment 23. Switch rod for lateral TV port 9. Lateral TV port 24. Transmitted light illumination 10. Coaxial coarse fine focus 25. Condenser 11. Fluorescence module 26. Transmitted light lamp housing 12. ICT prism adjustment 27. Transmitted light field diaphragm 13. Sextuple objective nosepiece 28. SLR port 14. Centring buttons for incident light 29. Second lamp housing 15. Field diaphragm adjustment INITIALISATION PROCEDURE 1. Ensure that both the DMIRE2 control box (CTRMIC) and the 50W mercury arc lamp power supply have been switched on (shown below). The CTRMIC control box can be found underneath the anti-vibration table that the DMIRE2 is sat upon (shown below); the 50W mercury lamp power supply is sat on the console bench. After switching on the CTR6000 control box the microscope will take a few seconds to initialize. The mercury arc lamp