Larval Peptides – A Novel Source of Antibiotics from ：一种新的源–幼虫肽抗生素.pptVIP

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2018-06-07 发布于浙江
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Larval Peptides – A Novel Source of Antibiotics from ：一种新的源–幼虫肽抗生素.ppt

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Larval Peptides – A Novel Source of Antibiotics from ：一种新的源–幼虫肽抗生素

Larval Peptides – A Novel Source of Antibiotics from Lucilia cuprina Maggots By: Tan Sheng En Troy (HCI) Choo Jian Kai Darren (HCI) Joanne Guidry (AOS) Robbie Daitzman (AOS) Introduction Insects possess a different immune system from Man Lack immunoglobulin Possess peptides with antibacterial activities Appear in insects after they are injured or challenged by bacteria (Boman. et al, 1974) Introduction Maggots thrive in bacteria filled environments. It is natural that they have an insect immunity system to defend themselves against pathogens Disrupts cytoplasmic cell membrane of bacteria – loss of K+ ions (Boman. et al, 1974) Objectives To study the antibacterial Properties of Peptides isolated from Lucilia cuprina maggots To identify the specific peptides which exhibits the antibacterial properties Rationale Antibacterial peptides found in Lucilia cuprina can be used on humans Not just natural but also presents a wider range of activity Wide range of peptides to use to counter all kinds of diseases and bacteria. (Nguyenn et al, 2007) Hypothesis The hemolymph of the Lucilia cuprina maggots contains peptides that have antibacterial properties Variables Independent Types of bacteria tested Concentration of peptides Dependant Presence of antibacterial Peptides Extent of Bacteria Inhibition Sequences of peptides Controlled Species of Maggot used Materials Required Maggots Peptide Extraction Kit SDSGel Liver Desiccate Pig’s Heart Gram Positive/Gram Negative bacteria Escherichia coli Pseudomonas Fluorescens Staphylococcus epidermidis Micrococcus luteus Methodology Extraction of Hemolymph SDSAnti-bacterial Screening Peptide extraction HPLC Hemolymph Extraction Capillary tube inserted through thoracic pleura of maggot Hemolymph collected from 500 maggots Sample diluted with buffer Store at - 20oC till use Peptide Purification 500 -1000μl maggot hemolymph collected Solid-phase extraction with CAT-SEC X cartridges Peptides with a molecular weight below 20