一种海洋的弧菌胞外多糖a101的抗生物膜作用分析-analysis of anti-biofilm effect of marine vibrio exopolysaccharide a101.docxVIP

达到 85％。最低生物膜消除浓度 MBEC 试验结果表明， A101 可以提高生物膜对抗生素的敏感性，使阿米卡星对生物膜细菌的杀灭作用提高 16 倍以上。A101 并不抑制游离铜绿假单胞菌 FRD1 的生长，相反地能够促进其生长。唯 一碳源实验结果表明 A101 不能作为碳源被 FRD1 利用。进一步研究发现，A101 可以防止 FRD1 菌体簇集，使其分散生长，从而能够更好地利用环境营养。这种 分散作用也正是 A101 抗生物膜作用的机制。结合相关研究报道，推测 A101 可能 是封闭了 FRD1 细胞表面某些菌体赖以相互结合的位点，阻碍了菌体之间的相互 作用，从而抑制了生物膜形成；而且，由于 A101 对菌体表面位点的结合力强于 细菌间的结合力，故可以破坏已经形成的生物膜。本文发现了一种弧菌胞外多糖 A101，该多糖不仅抑制生物膜的形成，还能 破坏已经形成的生物膜；并且其作用具有广谱性，对多种细菌，尤其是临床常见 的铜绿假单胞菌、金黄色葡萄球菌、表皮葡萄球菌的生物膜均有作用；A101 可 以增强抗生素对生物膜细菌的杀灭效果，具有这种作用的多糖在国际上尚属首次 报道。A101 有望开发成为高效、广谱、安全的抗生物膜制剂，不但在临床细菌 生物膜相关慢性感染的治疗、抗细菌生物膜新材料的研发等方面具有重要的应用 前景，而且在生物膜形成与放散、细菌细胞间的识别和相互作用等多个领域具有 重要的研究价值。关键词：细菌生物膜；细菌耐药；海洋细菌；弧菌；细菌胞外多糖Anti-biofilm effect of a novel exopolysaccharidefrom marine Vibrio sp QY101 AbstractIn the past, bacterial cells were regarded as individual organisms growing in planktonic populations. However, it is now recognized that many or perhaps most bacteria have a strong propensity to form multicellular, matrix-enclosed assemblies, or biofilms, which are found on surfaces throughout the biological world. Cells within a biofilm have a number of advantages over their planktonic counterparts, including protection against the immune system and predation by protozoa, enhanced ability to transfer genetic information, and enhanced resistance to antimicrobial agents and other stresses. Thus, biofilm formation complicates a variety of chronic infections, including the devastating pulmonary infections that are caused by Pseudomonas aeruginosa in cystic fibrosis patients and other opportunistic infections by this organism. According to a public announcement by US National Institute of Health, “biofilms are medically important, accounting for over 80% of microbial infections in the body”. These problems of sessile organisms predominate in most of the environmental, industrial, and medical problems and cause the interest of microbiologists. Yet bacterial biofilms remain poorly understood and the strategies for their control remain underdeveloped. Therefore,